Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/103607
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Type: | Journal article |
Title: | Direct reprogramming of mouse fibroblasts to neural stem cells by small molecules |
Author: | Han, Y.C. Lim, Y. Duffieldl, M.D. Li, H. Liu, J. Abdul Manaph, N.P. Yang, M. Keating, D.J. Zhou, X.F. |
Citation: | Stem Cells International, 2016; 2016:4304916-1-4304916-11 |
Publisher: | Hindawi Publishing Corporation |
Issue Date: | 2016 |
ISSN: | 1687-966X 1687-9678 |
Statement of Responsibility: | Yan-Chuang Han, Yoon Lim, Michael D. Duffieldl, Hua Li, Jia Liu, Nimshitha Pavathuparambil AbdulManaph, Miao Yang, Damien J. Keating, and Xin-Fu Zhou |
Abstract: | Although it is possible to generate neural stem cells (NSC) from somatic cells by reprogramming technologies with transcription factors, clinical utilization of patient-specific NSC for the treatment of human diseases remains elusive. The risk hurdles are associated with viral transduction vectors induced mutagenesis, tumor formation from undifferentiated stem cells, and transcription factors-induced genomic instability. Here we describe a viral vector-free and more efficient method to induce mouse fibroblasts into NSC using small molecules. The small molecule-induced neural stem (SMINS) cells closely resemble NSC in morphology, gene expression patterns, self-renewal, excitability, and multipotency. Furthermore, the SMINS cells are able to differentiate into astrocytes, functional neurons, and oligodendrocytes in vitro and in vivo.Thus, we have established a novelway to efficiently induce neural stemcells (iNSC) fromfibroblasts using only smallmolecules without altering the genome. Such chemical induction removes the risks associated with current techniques such as the use of viral vectors or the induction of oncogenic factors. This technique may, therefore, enable NSC to be utilized in various applications within clinical medicine. |
Rights: | © 2016 Yan-Chuang Han et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
DOI: | 10.1155/2016/4304916 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/535093 http://purl.org/au-research/grants/nhmrc/595937 |
Published version: | http://dx.doi.org/10.1155/2016/4304916 |
Appears in Collections: | Aurora harvest 7 Medicine publications |
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hdl_103607.pdf | Published version | 11.64 MB | Adobe PDF | View/Open |
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