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Type: Journal article
Title: OsPAP10c, a novel secreted acid phosphatase in rice, plays an important role in the utilization of external organic phosphorus
Author: Lu, L.
Qiu, W.
Gao, W.
Tyerman, S.
Shou, H.
Wang, C.
Citation: Plant Cell and Environment, 2016; 39(10):2247-2259
Publisher: Wiley-Blackwell
Issue Date: 2016
ISSN: 0140-7791
Statement of
Linghong Lu, Wenmin Qiu, Wenwen Gao, Stephen D. Tyerman, Huixia Shou, Chuang Wang
Abstract: Under phosphate (Pi ) starvation, plants increase the secretion of purple acid phosphatases (PAPs) into the rhizosphere to scavenge organic phosphorus (P) for plant use. To date, only a few members of the PAP family have been characterized in crops. In this study, we identified a novel secreted PAP in rice, OsPAP10c, and investigated its role in the utilization of external organic P. OsPAP10c belongs to a monocotyledon-specific subclass of Ia group PAPs and is specifically expressed in the epidermis/exodermis cell layers of roots. Both the transcript and protein levels of OsPAP10c are strongly induced by Pi starvation. OsPAP10c overexpression increased acid phosphatase (APase) activity by more than 10-fold in the culture media and almost fivefold in both roots and leaves under Pi -sufficient and Pi -deficient conditions. This increase in APase activity further improved the plant utilization efficiency of external organic P. Moreover, several APase isoforms corresponding to OsPAP10c were identified using in-gel activity assays. Under field conditions with three different Pi supply levels, OsPAP10c-overexpressing plants had significantly higher tiller numbers and shorter plant heights. This study indicates that OsPAP10c encodes a novel secreted APase that plays an important role in the utilization of external organic P in rice.
Keywords: Oryza sativa (rice); purple acid phosphatase (PAP)
Rights: © 2016 John Wiley & Sons Ltd
RMID: 0030050654
DOI: 10.1111/pce.12794
Grant ID:
Appears in Collections:Agriculture, Food and Wine publications

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