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https://hdl.handle.net/2440/104025
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Type: | Journal article |
Title: | Early CCR6 expression on B cells modulates germinal centre kinetics and efficient antibody responses |
Author: | Reimer, D. Lee, A. Bannan, J. Fromm, P. Kara, E. Comerford, I. McColl, S. Wiede, F. Mielenz, D. Körner, H. |
Citation: | Immunology and Cell Biology, 2017; 95(1):33-41 |
Publisher: | Nature Publishing |
Issue Date: | 2017 |
ISSN: | 0818-9641 1440-1711 |
Statement of Responsibility: | Dorothea Reimer, Adrian YS Lee, Jennifer Bannan, Phillip Fromm, Ervin E Kara, Iain Comerford, Shaun McColl, Florian Wiede, Dirk Mielenz and Heinrich Körner |
Abstract: | The CC-chemokine receptor 6 (CCR6) can be detected on naïve and activated B cells. Counterintuitively, its absence accelerates the appearance of germinal centres (GC) and increases the production of low-affinity antibodies. The detailed mechanism of CCR6 function during the humoral response has remained elusive but previously we identified a distinct CCR6(high) B cell population in vivo early after antigenic challenge. In this study, we defined this population specifically as early, activated pre-GC B cells. In accordance, we show that CCR6 is up-regulated rapidly within hours on protein or mRNA level after activation in vitro. Additionally, only activated B cells migrated specifically towards CCL20, the specific ligand for CCR6. Lack of CCR6 increased the dark zone/light zone ratio of GC and led to decreased antigen-specific IgG1 and IgG2a antibody generation in a B cell intrinsic manner in mixed bone marrow chimeras. In contrast, antigen-specific IgM responses were normal. Hence, CCR6 negatively regulates entry of activated, antigen-specific pre-GC B cells into the GC reaction. |
Keywords: | Germinal Center B-Lymphocytes Animals Mice, Inbred C57BL RNA, Messenger Flow Cytometry Lymphocyte Activation Cell Movement Antibody Formation Up-Regulation Kinetics Receptors, CCR6 Chemokine CCL20 |
Rights: | © 2017 Australasian Society for Immunology Inc. All rights reserved |
DOI: | 10.1038/icb.2016.68 |
Grant ID: | NHMRC |
Published version: | http://dx.doi.org/10.1038/icb.2016.68 |
Appears in Collections: | Aurora harvest 7 Molecular and Biomedical Science publications |
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