Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/105169
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Type: Journal article
Title: Effects ofs from Escherichia coli Nissle 1917 and Faecalibacterium prausnitzii on intestinal epithelial cells and a rat model of 5-fluorouracil-induced mucositis
Author: Wang, H.
Jatmiko, Y.
Bastian, S.
Mashtoub, S.
Howarth, G.
Citation: Nutrition and Cancer, 2017; 69(2):307-318
Publisher: Taylor & Francis
Issue Date: 2017
ISSN: 0163-5581
1532-7914
Statement of
Responsibility: 
Hanru Wang, Yoga D. Jatmiko, Susan E. P. Bastian, Suzanne Mashtoub & Gordon S. Howarth
Abstract: Faecalibacterium prausnitzii (Fp) and Escherichia coli Nissle 1917 (EcN) are probiotics, which have been reported to ameliorate certain gastrointestinal disorders. We evaluated the effects of supernatants (SN) derived from Fp and EcN on 5-fluorouracil (5-FU)-treated intestinal cells and in a rat model of mucositis. In vitro: IEC-6, Caco-2, and T-84 cells were analyzed for viability and monolayer permeability. In vivo: Female dark agouti rats were gavaged with Fp or EcN SN and injected intraperitoneally with saline (control) or 5-FU to induce mucositis. Rats were euthanized and intestinal tissues collected for myeloperoxidase assay and histological analyses. In vitro: Caco-2 cell viability was further reduced when treated with Fp SN + 5-FU compared to 5-FU controls. In both Caco-2 and T-84 cells, Fp SN partially prevented the decrease in transepithelial electrical resistance (TER) caused by 5-FU administration. In vivo: 5-FU-injected rats administered Fp SN or EcN SN partly prevented body weight loss and normalized water intake compared to 5-FU controls. These results suggest a growth inhibitory mechanism of Fp SN action on transformed epithelial cells that could be mediated by effects on tight junctions. Factors derived from Fp SN and EcN SN could have a role in reducing the severity of intestinal mucositis.
Keywords: Intestines; Caco-2 Cells; Epithelial Cells; Animals; Humans; Rats; Escherichia coli; Fluorouracil; Anti-Inflammatory Agents, Non-Steroidal; Immunologic Factors; Culture Media, Conditioned; Cell Survival; Probiotics; Female; Mucositis; Faecalibacterium prausnitzii
Rights: © 2017 Taylor & Francis Group, LLC
RMID: 0030062408
DOI: 10.1080/01635581.2017.1263747
Appears in Collections:Animal and Veterinary Sciences publications

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