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https://hdl.handle.net/2440/10629
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Type: | Journal article |
Title: | Downregulation of amyloid precursor protein (APP) expression following post-traumatic cyclosporin-A administration |
Author: | Van Den Heuvel, C. Donkin, J. Finnie, J. Blumbergs, P. Kuchel, T. Koszyca, B. Manavis, J. Jones, N. Reilly, P. Vink, R. |
Citation: | Journal of Neurotrauma, 2004; 21(11):1562-1572 |
Publisher: | Mary Ann Liebert Inc Publ |
Issue Date: | 2004 |
ISSN: | 0897-7151 1557-9042 |
Statement of Responsibility: | Corinna Van Den Heuvel, James J. Donkin, John W. Finnie, Peter C. Blumbergs, Tim Kuchel, Barbara Koszyca, Jim Manavis, Nigel R. Jones, Peter L. Reilly, Robert Vink |
Abstract: | The aim of these studies was to assess and quantitate the effects of cyclosporin-A (CyA) on brain APP messenger RNA and neuronal perikaryal APP antigen expression following controlled focal head impact in sheep. Impact results in a significant increase in both APP mRNA and neuronal perikaryal APP antigen expression. Post-traumatic administration of CyA (intrathecal 10 mg/kg) resulted in a reduction in APP mRNA and neuronal perikaryal antigen expression. At 2 h postinjury, CyA treatment caused a statistically significant (p < 0.05) 1.3 +/- 0.1-fold decrease in APP mRNA in the central gray matter of impacted sheep compared to untreated impacted sheep. A more profound reduction in APP mRNA synthesis (1.6 +/- 0.2 fold) was evident at 6 h (p < 0.05). The mean percentage brain area with APP immunoreactive neuronal perikarya at 6 h post-injury was 94.5% in untreated impacted animals, 10.0% in CyA-treated impacted animals, 5.5% in untreated nonimpacted animals, and 6% in CyA-treated non-impacted controls. These results demonstrate that CyA has a downregulatory effect on increased APP expression caused by TBI. |
Keywords: | Brain Animals Sheep Brain Injuries Disease Models, Animal Nerve Degeneration Cyclosporine Amyloid beta-Protein Precursor RNA, Messenger Neuroprotective Agents Immunohistochemistry Injections, Spinal Down-Regulation Up-Regulation Time Factors Female |
Description: | Copyright © 2004 Mary Ann Liebert, Inc. |
DOI: | 10.1089/neu.2004.21.1562 |
Published version: | http://dx.doi.org/10.1089/neu.2004.21.1562 |
Appears in Collections: | Aurora harvest 2 Surgery publications |
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