Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/106988
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Viperin is an important host restriction factor in control of Zika virus infection |
Author: | Van Der Hoek, K. Eyre, N. Shue, B. Khantisitthiporn, O. Glab-Ampi, K. Carr, J. Gartner, M. Jolly, L. Thomas, P. Adikusuma, F. Jankovic-Karasoulos, T. Roberts, C. Helbig, K. Beard, M. |
Citation: | Scientific Reports, 2017; 7(1):4475 -1-4475-14 |
Publisher: | Nature Publishing Group |
Issue Date: | 2017 |
ISSN: | 2045-2322 2045-2322 |
Statement of Responsibility: | Kylie H. Van der Hoek, Nicholas S. Eyre, Byron Shue, Onruedee Khantisitthiporn, Kittirat Glab-Ampi, Jillian M. Carr, Matthew J. Gartner, Lachlan A. Jolly, Paul Q. Thomas, Fatwa Adikusuma, Tanja Jankovic-Karasoulos, Claire T. Roberts, Karla J. Helbig and Michael R. Beard |
Abstract: | Zika virus (ZIKV) infection has emerged as a global health threat and infection of pregnant women causes intrauterine growth restriction, spontaneous abortion and microcephaly in newborns. Here we show using biologically relevant cells of neural and placental origin that following ZIKV infection, there is attenuation of the cellular innate response characterised by reduced expression of IFN-β and associated interferon stimulated genes (ISGs). One such ISG is viperin that has well documented antiviral activity against a wide range of viruses. Expression of viperin in cultured cells resulted in significant impairment of ZIKV replication, while MEFs derived from CRISPR/Cas9 derived viperin-/- mice replicated ZIKV to higher titers compared to their WT counterparts. These results suggest that ZIKV can attenuate ISG expression to avoid the cellular antiviral innate response, thus allowing the virus to replicate unchecked. Moreover, we have identified that the ISG viperin has significant anti-ZIKV activity. Further understanding of how ZIKV perturbs the ISG response and the molecular mechanisms utilised by viperin to suppress ZIKV replication will aid in our understanding of ZIKV biology, pathogenesis and possible design of novel antiviral strategies. |
Keywords: | Monocytes Cell Line Macrophages Placenta Animals Mice, Knockout Humans Mice Disease Models, Animal Oxidoreductases Acting on CH-CH Group Donors Proteins Virus Replication Pregnancy Female Host-Pathogen Interactions Immunity, Innate Neural Stem Cells CRISPR-Cas Systems Zika Virus Zika Virus Infection Gene Editing |
Description: | Published online 30 June 2017 |
Rights: | © The Author(s) 2017. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
DOI: | 10.1038/s41598-017-04138-1 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1053206 |
Published version: | http://dx.doi.org/10.1038/s41598-017-04138-1 |
Appears in Collections: | Aurora harvest 3 Paediatrics publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_106988.pdf | Published Version | 5.38 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.