Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/107260
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dc.contributor.authorAmalraj, A.en
dc.contributor.authorLuang, S.en
dc.contributor.authorKumar, M.en
dc.contributor.authorSornaraj, P.en
dc.contributor.authorEini, O.en
dc.contributor.authorKovalchuk, N.en
dc.contributor.authorBazanova, N.en
dc.contributor.authorLi, Y.en
dc.contributor.authorYang, N.en
dc.contributor.authorEliby, S.en
dc.contributor.authorLangridge, P.en
dc.contributor.authorHrmova, M.en
dc.contributor.authorLopato, S.en
dc.date.issued2016en
dc.identifier.citationPlant Biotechnology Journal, 2016; 14(2):820-832en
dc.identifier.issn1467-7644en
dc.identifier.issn1467-7652en
dc.identifier.urihttp://hdl.handle.net/2440/107260-
dc.descriptionFirst published: 6 July 2015en
dc.description.abstractPlants respond to abiotic stresses by changes in gene regulation, including stress-inducible expression of transcriptional activators and repressors. One of the best characterized families of drought-related transcription factors are dehydration-responsive element binding (DREB) proteins, known as C-repeat binding factors (CBF). The wheat DREB/CBF gene TaRAP2.1L was isolated from drought-affected tissues using a dehydration-responsive element (DRE) as bait in a yeast one-hybrid screen. TaRAP2.1L is induced by elevated abscisic acid, drought and cold. A C-terminal ethylene responsive factor-associated amphiphilic repression (EAR) motif, known to be responsible for active repression of target genes, was identified in the TaRAP2.1L protein. It was found that TaRAP2.1L has a unique selectivity of DNA-binding, which differs from that of DREB activators. This binding selectivity remains unchanged in a TaRAP2.1L variant with an inactivated EAR motif (TaRAP2.1Lmut). To study the role of the TaRAP2.1L repressor activity associated with the EAR motif in planta, transgenic wheat overexpressing native or mutated TaRAP2.1L was generated. Overexpression of TaRAP2.1L under constitutive and stress-inducible promoters in transgenic wheat and barley led to dwarfism and decreased frost tolerance. By contrast, constitutive overexpression of the TaRAP2.1Lmut gene had little or no negative influence on wheat development or grain yield. Transgenic lines with the TaRAP2.1Lmut transgene had an enhanced ability to survive frost and drought. The improved stress tolerance is attributed to up-regulation of several stress-related genes known to be downstream genes of DREB/CBF activators.en
dc.description.statementofresponsibilityAmritha Amalraj, Sukanya Luang, Manoj Yadav Kumar, Pradeep Sornaraj, Omid Eini, Nataliya Kovalchuk, Natalia Bazanova, Yuan Li, Nannan Yang, Serik Eliby, Peter Langridge, Maria Hrmova and Sergiy Lopatoen
dc.language.isoenen
dc.publisherWileyen
dc.rights© 2015 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltden
dc.subjectEAR motif; dehydration-responsive element binding proteins; drought; frost; transcriptional repressor; transgenic wheaten
dc.titleChange of function of the wheat stress-responsive transcriptional repressor TaRAP2.1L by repressor motif modificationen
dc.typeJournal articleen
dc.identifier.rmid0030031668en
dc.identifier.doi10.1111/pbi.12432en
dc.identifier.pubid193393-
pubs.library.collectionAgriculture, Food and Wine publicationsen
pubs.library.teamDS05en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidAmalraj, A. [0000-0003-0441-1192]en
dc.identifier.orcidLangridge, P. [0000-0001-9494-400X]en
dc.identifier.orcidHrmova, M. [0000-0002-3545-0605]en
Appears in Collections:Agriculture, Food and Wine publications

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