Non-selective cation channel activity of aquaporin AtPIP2;1 regulated by Ca²⁺ and pH

Abstract

The aquaporin AtPIP2;1 is an abundant plasma membrane intrinsic protein in Arabidopsis thaliana implicated in stomatal closure, but is also highly expressed in plasma membranes of root epidermal cells. When expressed in Xenopus laevis oocytes, AtPIP2;1 increased water permeability and induced a non-selective cation conductance mainly associated with Na⁺. A mutation in the water pore, G103W, prevented both the ionic conductance and water permeability of PIP2;1. Co-expression of AtPIP2;1 with AtPIP1;2 increased water permeability but abolished the ionic conductance. AtPIP2;2 (93% identical to AtPIP2;1) similarly increased water permeability but not ionic conductance. The ionic conductance was inhibited by the application of extracellular Ca²⁺ and Cd²⁺, with Ca²⁺ giving a biphasic dose-response with a prominent IC50 of 0.32 mм comparable to a previous report of Ca²⁺ -sensitivity of a non-selective cation channel (NSCC) in Arabidopsis root protoplasts. Low external pH also inhibited ionic conductance (IC50 pH 6.8). Xenopus oocytes and Saccaromyces cerevisiae expressing AtPIP2;1 accumulated more Na⁺ than controls. Establishing whether AtPIP2;1 has dual ion and water permeability in planta will be important in understanding the roles of this aquaporin, and if AtPIP2;1 is a candidate for a previously reported NSCC responsible for Ca²⁺ and pH sensitive Na⁺ entry into roots.