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dc.contributor.authorHynes, K.en
dc.contributor.authorBright, R.en
dc.contributor.authorMarino, V.en
dc.contributor.authorNg, J.en
dc.contributor.authorVerma, P.en
dc.contributor.authorGronthos, S.en
dc.contributor.authorBartold, P.en
dc.identifier.citationStem Cells International, 2018; 2018:2601945-1-2601945-12en
dc.description.abstractMesenchymal stromal cell-like populations have been derived from mouse-induced pluripotent stem cells (miPSC-MSC) with the capability for tissue regeneration. In this study, murine iPSC underwent differentiation towards an MSC-like immunophenotype. Stable miPSC-MSC cultures expressed the MSC-associated markers, CD73, CD105, and Sca-1, but lacked expression of the pluripotency marker, SSEA1, and hematopoietic markers, CD34 and CD45. Functionally, miPSC-MSC exhibited the potential for trilineage differentiation into osteoblasts, adipocytes, and chondrocytes and the capacity to suppress the proliferation of mitogen-activated splenocytes. The efficacy of miPSC-MSC was assessed in an acute inflammation model following systemic or local delivery into mice with subcutaneous implants containing heat-inactivated P. gingivalis. Histological analysis revealed less inflammatory cellular infiltrate within the sponges in mice treated with miPSC-MSC cells delivered locally rather than systemically. Assessment of proinflammatory cytokines in mouse spleens found that CXCL1 transcripts and protein were reduced in mice treated with miPSC-MSC. In a periodontitis model, mice subjected to oral inoculation with P. gingivalis revealed less bone tissue destruction and inflammation within the jaws when treated with miPSC-MSC compared to PBS alone. Our results demonstrated that miPSC-MSC derived from iPSC have the capacity to control acute and chronic inflammatory responses associated with the destruction of periodontal tissue. Therefore, miPSC-MSC present a promising novel source of stromal cells which could be used in the treatment of periodontal disease and other inflammatory systemic diseases such as rheumatoid arthritis.en
dc.description.statementofresponsibilityK. Hynes, R. Bright, V. Marino, J. Ng, P. J. Verma, S. Gronthos and P. M. Bartolden
dc.publisherHindawi Publishing Corporationen
dc.rightsCopyright © 2018 K. Hynes et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.titlePotential of iPSC-derived mesenchymal stromal cells for treating periodontal diseaseen
dc.typeJournal articleen
pubs.library.collectionDentistry publicationsen
dc.identifier.orcidNg, J. [0000-0003-2302-2643]en
dc.identifier.orcidGronthos, S. [0000-0002-6225-3084]en
dc.identifier.orcidBartold, P. [0000-0002-5695-3877]en
Appears in Collections:Dentistry publications

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