Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/112607
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Type: | Journal article |
Title: | Combining experimental evolution with next-generation sequencing: a powerful tool to study adaptation from standing genetic variation |
Author: | Schlötterer, C. Kofler, R. Versace, E. Tobler, R. Franssen, S. |
Citation: | Heredity, 2015; 114(5):431-440 |
Publisher: | Springer Nature |
Issue Date: | 2015 |
ISSN: | 0018-067X 1365-2540 |
Statement of Responsibility: | C Schlötterer, R Kofler, E Versace, R Tobler and SU Franssen |
Abstract: | Evolve and resequence (E&R) is a new approach to investigate the genomic responses to selection during experimental evolution. By using whole genome sequencing of pools of individuals (Pool-Seq), this method can identify selected variants in controlled and replicable experimental settings. Reviewing the current state of the field, we show that E&R can be powerful enough to identify causative genes and possibly even single-nucleotide polymorphisms. We also discuss how the experimental design and the complexity of the trait could result in a large number of false positive candidates. We suggest experimental and analytical strategies to maximize the power of E&R to uncover the genotype–phenotype link and serve as an important research tool for a broad range of evolutionary questions. |
Keywords: | Animals Drosophila melanogaster Adaptation, Physiological Gene Frequency Genotype Phenotype Polymorphism, Single Nucleotide Research Design Genetic Variation Genetic Association Studies Biological Evolution High-Throughput Nucleotide Sequencing |
Rights: | © 2015 Macmillan Publishers Limited All rights reserved This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ |
DOI: | 10.1038/hdy.2014.86 |
Published version: | http://dx.doi.org/10.1038/hdy.2014.86 |
Appears in Collections: | Aurora harvest 3 Genetics publications |
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hdl_112607.pdf | Published Version | 683.13 kB | Adobe PDF | View/Open |
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