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Type: Journal article
Title: DNA binding by the coliphage 186 repressor protein C1
Author: Dodd, I.
Egan, J.
Citation: Journal of Biological Chemistry, 1996; 271(19):11532-11540
Issue Date: 1996
ISSN: 0021-9258
Abstract: The cI gene of coliphage 186 maintains lysogeny and confers immunity to 186 infection by repressing the major early promoter, p(R), and the promoter for the late transcription activator gene, p(B). Gel mobility shirt and DNase I footprinting show that CI protein binds to the DNA at p(R) and p(B) and also to sites approximately 300 base pairs upstream and downstream of p(R), called FL and FR. Mutations which cause virulence reduce CI binding to p(R). The biochemical and genetic data identify three CI operators at p(R), two at p(B), and single operators at FL and FR. The operators at the p(B), FL, FR, and central p(R) sites are inverted repeat sequences, separated by 5 base pairs (Type A) or, in the case of p(R), by 4 base pairs (Type A'). A different inverted repeat operator sequence (Type B) is proposed for the binding sites on each side of the central site at p(R). Thus, CI appears to recognize two distinct DNA sequences. CI binds cooperatively to adjacent operators, and binding at p(R) is strongly dependent on these cooperative interactions. A high order CI multimer appears to be the active DNA binding species, even at single operators.
Keywords: Escherichia coli
Deoxyribonuclease I
DNA-Binding Proteins
Repressor Proteins
Viral Proteins
DNA Primers
DNA Footprinting
Polymerase Chain Reaction
Base Composition
Binding Sites
Amino Acid Sequence
Base Sequence
Repetitive Sequences, Nucleic Acid
Consensus Sequence
Genes, Fungal
Molecular Sequence Data
Viral Regulatory and Accessory Proteins
Promoter Regions, Genetic
DOI: 10.1074/jbc.271.19.11532
Appears in Collections:Aurora harvest 2
Biochemistry publications

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