Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: The CII protein of bacteriophage 186 establishes lysogeny by activating a promoter upstream of the lysogenic promoter
Author: Neufing, P.
Shearwin, K.
Camerotto, J.
Egan, J.
Citation: Molecular Microbiology, 1996; 21(4):751-761
Publisher: Blackwell Science
Issue Date: 1996
ISSN: 0950-382X
Statement of
Petra J. Neufing, Keith E. Shearwin, Julianne Camerotto and J. Barry Egan
Abstract: We have shown previously that the cII gene product of the non-lambdoid temperate bacteriophage 186 is required for the establishment of lysogeny. We show here that CII, a potential helix-turn-helix DNA-binding protein, establishes lysogeny by activating a promoter (p E) which spans the apl/cII intergenic region, upstream of the lysogenic promoter, p L. The start site of the p E transcript (+1) has been mapped by primer extension and we have identified the CII binding determinants at p E by DNase I footprinting. CII binds to inverted repeat sequences separated by two turns of the helix, with binding half-sites centred at the −38 and −58 positions of p E. Oligomerisation studies with purified CII protein indicate that a CII tetramer may be the species that binds to this site. We also show that p E is subject to direct negative feedback by the CI repressor.
Description: Article first published online: 31 OCT 2003
Rights: © 1996 Blackwell Science Ltd.
DOI: 10.1046/j.1365-2958.1996.351394.x
Appears in Collections:Aurora harvest 7
Biochemistry publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.