Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/113516
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Type: Journal article
Title: Disposable microfluidic micromixers for effective capture of Cryptosporidium parvum oocysts from water samples
Author: Diéguez, L.
Winter, M.
Molan, S.
Monis, P.
King, B.
Thierry, B.
Citation: Journal of Biological Engineering, 2018; 12(1):4-1-4-8
Publisher: BioMed Central
Issue Date: 2018
ISSN: 1754-1611
Statement of
Responsibility: 
L. Diéguez, M. Winter, S. Molan, P. Monis, B. King, and B. Thierry
Abstract: Background: Protecting drinking water supplies from pathogens such as Cryptosporidium parvum is a major concern for water utilities worldwide. The sensitivity and specificity of current detection methods are largely determined by the effectiveness of the concentration and separation methods used. The purpose of this study is to develop micromixers able to specifically isolate and concentrate Cryptosporidium, while allowing in situ analysis. Results: In this study, disposable microfluidic micromixers were fabricated to effectively isolate Cryptosporidium parvum oocysts from water samples, while allowing direct observation and enabling quantification of oocysts captured in the device using high quality immunofluorescence microscopy. In parallel, quantitative analysis of the capture yield was carried out by analyzing the waste from the microfluidics outlet with an Imaging Flow Cytometer. At the optimal flow rate, capture efficiencies up to 96% were achieved in spiked samples. Conclusions: Scaled microfluidic isolation and detection of Cryptosporidium parvum will provide a faster and more efficient detection method for Cryptosporidium compared to other available laboratory-scale technologies.
Keywords: Cryptosporidium parvum oocysts; disposable microfluidic micromixers; imaging flow cytometry, water quality; immunocytochemistry; fluorescence microscopy
Rights: © The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
RMID: 0030084904
DOI: 10.1186/s13036-018-0095-6
Grant ID: http://purl.org/au-research/grants/nhmrc/1045841
Appears in Collections:Microbiology and Immunology publications

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