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https://hdl.handle.net/2440/11407
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Type: | Journal article |
Title: | A repetitive DNA sequence common to the different B chromosomes of the genus Brachycome |
Author: | Houben, A. Leach, C. Verlin, D. Rofe, R. Timmis, J. |
Citation: | Chromosoma: biology of the nucleus, 1997; 106(8):513-519 |
Publisher: | SPRINGER VERLAG |
Issue Date: | 1997 |
ISSN: | 0009-5915 1432-0886 |
Statement of Responsibility: | Houben, Andreas ; Leach, Carolyn R. ; Verlin, Dawn ; Rofe, Ruth ; Timmis, Jeremy N. |
Abstract: | Dot-like micro B chromosomes of Brachycome dichromosomatica were analysed for their sequence composition. Southern hybridization patterns of a total micro B probe to genomic DNA from plants with and without micro Bs demonstrated that the micro Bs shared sequences with the A chromosomes. In addition to telomere, rDNA and common A and B chromosome sequences, a new B-specific, highly methylated tandem repeat (Bdm29) was detected. After in situ hybridization with Bdm29 the entire micro B chromosome was labelled and clustering of the condensed micro Bs could be observed at interphase. A high number of Bdm29-like sequences were also found in the larger B chromosomes of B. dichromosomatica and in other Bs within the genus Brachycome. |
Keywords: | Chromosomes Telomere Plants Plant Leaves DNA, Plant Base Sequence Repetitive Sequences, Nucleic Acid Molecular Sequence Data |
DOI: | 10.1007/PL00007689 |
Published version: | http://dx.doi.org/10.1007/pl00007689 |
Appears in Collections: | Aurora harvest 7 Genetics publications |
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