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Type: Journal article
Title: A repetitive DNA sequence common to the different B chromosomes of the genus Brachycome
Author: Houben, A.
Leach, C.
Verlin, D.
Rofe, R.
Timmis, J.
Citation: Chromosoma, 1997; 106(8):513-519
Issue Date: 1997
ISSN: 0009-5915
Statement of
Houben, Andreas ; Leach, Carolyn R. ; Verlin, Dawn ; Rofe, Ruth ; Timmis, Jeremy N.
Abstract: Dot-like micro B chromosomes of Brachycome dichromosomatica were analysed for their sequence composition. Southern hybridization patterns of a total micro B probe to genomic DNA from plants with and without micro Bs demonstrated that the micro Bs shared sequences with the A chromosomes. In addition to telomere, rDNA and common A and B chromosome sequences, a new B-specific, highly methylated tandem repeat (Bdm29) was detected. After in situ hybridization with Bdm29 the entire micro B chromosome was labelled and clustering of the condensed micro Bs could be observed at interphase. A high number of Bdm29-like sequences were also found in the larger B chromosomes of B. dichromosomatica and in other Bs within the genus Brachycome.
Keywords: Chromosomes; Telomere; Plants; Plant Leaves; DNA, Plant; Base Sequence; Repetitive Sequences, Nucleic Acid; Molecular Sequence Data
RMID: 0030004334
DOI: 10.1007/PL00007689
Appears in Collections:Genetics publications

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