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|Title:||The molecular organisation of a B chromosome tandem repeat sequence from Brachycome dichromosomatica|
|Citation:||Chromosoma, 1996; 105(4):223-230|
|Franks, T. ; Houben, A. ; Leach, C. ; Timmis, J.|
|Abstract:||A high copy, tandemly repeated, sequence (Bd49) specific to the B chromosome and located near the centromere in Brachycome dichromosomatica was used to identify lambda genomic clones from DNA of a 3B plant. Only one clone of those analysed was composed entirely of a tandem array of the B-specific repeat unit. In other clones, the Bd49 repeats were linked to, or interspersed with, sequences that are repetitious and distributed elsewhere on the A and B chromosomes. One such repetitious flanking sequence has similarity to retrotransposon sequences and a second is similar to chloroplast DNA sequences. Of the four separate junctions analysed of Bd49-like sequence with flanking sequence, three were associated with the same A/T-rich region in Bd49 and the fourth was close to a 25 bp imperfect dyadic sequence. No novel B-specific sequences were detected within the genomic clones.|
|Keywords:||Chromosomes; Plants; Deoxyribonucleases, Type II Site-Specific; DNA, Plant; Blotting, Southern; Cloning, Molecular; Polymerase Chain Reaction; Sequence Analysis, DNA; Base Sequence; Repetitive Sequences, Nucleic Acid; Sequence Homology, Nucleic Acid; Molecular Sequence Data|
|Appears in Collections:||Genetics publications|
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