Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/114132
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Type: | Journal article |
Title: | miR-142-5p in bone marrow-derived Mesenchymal stem cells promotes osteoporosis involving targeting adhesion molecule VCAM-1 and inhibiting cell migration |
Author: | Teng, Z. Xie, X. Zhu, Y. Liu, J. Hu, X. Na, Q. Zhang, X. Wei, G. Xu, S. Liu, Y. Zhang, X. Xian, C.J. |
Citation: | BioMed Research International, 2018; 2018:3274641-1-3274641-7 |
Publisher: | Hindawi |
Issue Date: | 2018 |
ISSN: | 2314-6133 2314-6141 |
Statement of Responsibility: | Zhaowei Teng, Xueguan Xie, Yun Zhu, Jianping Liu, Xingbo Hu, Qiang Na, Xiongwen Zhang, Guojun Wei, Shen Xu, Yugang Liu, Xiguang Zhang and Cory J. Xian |
Abstract: | Osteoporosis is a systemic bone metabolic disease that is highly prevalent in the elderly population, particularly in postmenopausal women, which results in enhanced bone fragility and an increased susceptibility to fractures. However, the underlying molecular pathogenesis mechanisms still remain to be further elucidated. In this study, in a rat ovariectomy- (OVX-) induced postmenopausal osteoporosis model, aberrant expression of a microRNA miR-142-5p and vascular cell adhesion molecule 1 (VCAM-1) was found by RNA sequencing analysis and qRT-PCR. Using a dual-luciferase reporter assay, we found that miR-142-5p can bind to and decrease expression of VCAM-1 mRNA. Such reduction was prohibited when the miR-142-5p binding site in VCAM-1 3'UTR was deleted, and Western blotting analyses validated the fact that miR-142-5p inhibited the expression of VCAM-1 protein. Bone marrow-derived mesenchymal stem cells (BMMSCs) transfected with miR-142-5p showed a significantly decreased migration ability in a Transwell migration assay. Collectively, these data indicated the important role of miR-142-5p in osteoporosis development involving targeting VCAM-1 and inhibiting BMMSC migration. |
Keywords: | Bone Marrow Cells Mesenchymal Stem Cells Animals Rats Rats, Sprague-Dawley Osteoporosis Vascular Cell Adhesion Molecule-1 MicroRNAs 3' Untranslated Regions Cell Movement Gene Expression Regulation Female |
Rights: | © 2018 Zhaowei Teng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
DOI: | 10.1155/2018/3274641 |
Grant ID: | 81660156 81760136 81671928 2014FZ048 2017FE468-181 http://purl.org/au-research/grants/nhmrc/1042105 |
Published version: | http://dx.doi.org/10.1155/2018/3274641 |
Appears in Collections: | Aurora harvest 8 Medicine publications |
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hdl_114132.pdf | Published version | 2.99 MB | Adobe PDF | View/Open |
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