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Type: Journal article
Title: Genomic characterization of coagulase-negative staphylococci including methicillin-resistant Staphylococcus sciuri causing bovine mastitis
Author: Khazandi, M.
Al-Farha, A.
Coombs, G.
O'Dea, M.
Pang, S.
Trott, D.
Aviles, R.
Hemmatzadeh, F.
Venter, H.
Ogunniyi, A.
Hoare, A.
Abraham, S.
Petrovski, K.
Citation: Veterinary Microbiology, 2018; 219:17-22
Publisher: Elsevier
Issue Date: 2018
ISSN: 0378-1135
Statement of
Manouchehr Khazandi, Abd Al-Bar Al-Farha, Geoffrey W. Coombs, Mark O, Dea, Stanley Pang, Darren J. Trott, Ricardo R. Aviles, Farhid Hemmatzadeh, Henrietta Venter, Abiodun D. Ogunniyi, Andrew Hoare, Sam Abraham, Kiro R. Petrovski
Abstract: Methicillin-resistant coagulase-negative staphylococci (MRCoNS) have recently emerged as a significant cause of bovine mastitis worldwide. Here we describe the isolation of MRCoNS from cases of bovine mastitis from a single dairy farm in Australia. Fourteen CoNS isolates were identified as MRCoNS on the basis of having an oxacillin MIC of ≥0.5 μg/mL. The isolates were speciated as S. chromogenes (n = 1) S. fleurettii (n = 1), S. haemolyticus (n = 2), S. sciuri (n = 5), S. simulans (n = 1) S. succinus (n = 2) and S. xylosus (n = 2). Five of the isolates (S. fleuretti, S. haemolyticus S. sciuri and two S. succinus) were mecA-positive. We also detected a previously described S. sciuri mecA homolog in four oxacillin-resistant S. sciuri isolates. The remainder of the putative MRCoNS did not contain any mecA-related resistance determinants in their genomes. Comparative genomic analysis of three previously published S. sciuri isolates, from humans, a squirrel and a cereal crop (rice), and a representative isolate from our study demonstrated clustering and a high degree of genetic homogeneity (>95%), suggesting S. sciuri has low host specificity. In conclusion, CoNS, in particular S. sciuri, may act as a reservoir for SCCmec elements that can easily be spread between different host species by direct cross-infection.
Keywords: Dairy cattle
Methicillin-resistant coagulase-negative staphylococci
Staphylococcus sciuri
Whole genome sequencing
Rights: © 2018 Elsevier B.V. All rights reserved.
DOI: 10.1016/j.vetmic.2018.04.004
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Appears in Collections:Animal and Veterinary Sciences publications
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