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|Title:||The stoned locus of drosophila melanogaster produces a dicistronic transcript and encodes two distinct polypeptides|
|Citation:||Genetics, 1996; 143(4):1699-1711|
|Publisher:||Genetics Society of America|
|Justen Andrews, Michiko Smith, John Merakovsky, Michelle Coulson, Frances Hannan and Leonard E. Kelly|
|Abstract:||The stoned gene of Drosophila melanogaster is required for normal neuronal function in both adult and larva. We have identified DNA sequences that lie within a genetic region that is known to include the stoned gene and that also reveal restriction site variations in two stoned lethal mutants. This genomic region contains a single transcription unit coding for an ~8.4-kb transcript. The transcript is preferentially expressed in the head of adult flies. The isolation and sequencing of cDNA and genomic clones reveals that stoned appears to encode a dicistronic mRNA, although the possible existence of other forms of mRNA cannot be excluded. Antibody cross-reactivity shows that two proteins are translated from the stoned locus in vivo. Both open reading frames (ORFs) encode novel proteins. The protein encoded by the first ORF contains four tandemly repeated motifs, and one domain of the protein encoded by the second ORF shows similarity to a family of proteins (AP50s) associated with clathrin assembly protein complexes.|
|Keywords:||Animals; Drosophila melanogaster; Proteins; DNA, Complementary; RNA, Messenger; Immunochemistry; Restriction Mapping; Cloning, Molecular; Genetic Complementation Test; Transcription, Genetic; Amino Acid Sequence; Base Sequence; Sequence Homology, Amino Acid; Genes, Insect; Open Reading Frames; Molecular Sequence Data; Female; Male; Nervous System Physiological Phenomena|
|Description:||Copyright © 1996 by the Genetics Society of America|
|Appears in Collections:||Genetics publications|
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