Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/11436
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dc.contributor.authorDonald, T.en
dc.contributor.authorHouben, A.en
dc.contributor.authorLeach, C.en
dc.contributor.authorTimmis, J.en
dc.date.issued1997en
dc.identifier.citationGenome, 1997; 40(5):674-681en
dc.identifier.issn0831-2796en
dc.identifier.issn1480-3321en
dc.identifier.urihttp://hdl.handle.net/2440/11436-
dc.description© 1997 NRC Canadaen
dc.description.abstractRibosomal RNA genes are present near the end of the short arm and, to a lesser extent, near the centromere of the B chromosomes of some populations of Brachycome dichromosomatica. The internal transcribed spacer (ITS2) was amplified by PCR from total leaf DNA using primers within the conserved regions encoding the 5.8S and 25S stable rRNA species. Comparison of PCR amplified ITS2 sequences from several individual plants without B chromosomes with corresponding sequences derived from microdissected B chromosomes revealed two consistent differences between the rDNA of A and B chromosomes. One of these differences produced an SfcI restriction site that was present only in the ITS2 of the B-chromosome rDNA. Amplification by PCR of ITS2 from total genomic DNA from plants with and without B chromosomes showed an additive relationship between the amount of PCR product containing the SfcI site and the number of B chromosomes present. Quantitative analysis indicated that the proportion of total nuclear rDNA present on a single B chromosome varied between 2 and 4% in different A chromosome backgrounds. Similar experiments, with appropriate positive and negative controls, using reverse transcriptase PCR of the equivalent region within the 40S precursor rRNA, suggested that the B-chromosome rDNA was not transcribed. Similarly, PCR of reverse transcribed total RNA from plants containing B chromosomes using primers specific for the B chromosome ITS2 was unable to detect a transcript from the B chromosome. Keywords: B chromosome, ribosomal RNA genes, transcription.en
dc.description.statementofresponsibilityTamzin M. Donald, Andreas Houben, Carolyn R. Leach, and Jeremy N. Timmisen
dc.language.isoenen
dc.publisherNATL RESEARCH COUNCIL CANADAen
dc.subjectChromosomes; Plants; Plant Leaves; RNA, Plant; RNA, Ribosomal; Transcription, Genetic; Base Sequence; Molecular Sequence Dataen
dc.titleRibosomal RNA genes specific to the B chromosomes in Brachycome dichromosomatica are not transcribed in leaf tissueen
dc.typeJournal articleen
dc.identifier.rmid0030004314en
dc.identifier.doi10.1139/g97-089en
dc.identifier.pubid68320-
pubs.library.collectionGenetics publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
Appears in Collections:Genetics publications

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