Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/11451
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Type: Journal article
Title: Construction of a 1-Mb restriction-mapped cosmid contig containing the candidate region for the Familial Mediterranean Fever Locus (MEFV) on chromosome 16p13.3
Author: Sood, R.
Blake, T.
Aksentijevich, I.
Wood, G.
Chen, X.
Gardner, D.
Shelton, D.
Mangelsdorf, M.
Orsborn, A.
Pras, E.
Balow, J.
Centola, M.
Deng, Z.
Zaks, N.
Chen, X.
Richards, N.
Fischel-Ghodsian, N.
Rotter, J.
Pras, M.
Shohat, M.
et al.
Citation: Genomics, 1997; 42(1):83-95
Publisher: ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
Issue Date: 1997
ISSN: 0888-7543
1089-8646
Statement of
Responsibility: 
Sood, Raman; Blake, Trevor; Aksentijevich, Ivona; Wood, Geryl; Chen, Xiang; Gardner, Dawn; Shelton, David A.; Mangelsdorf, Marie; Orsborn, Annette; Pras, Elon; Balow, James E.; Centola, Michael; Deng, Zuoming; Zaks, Nurit; Chen, Xiaoguang; Richards, Neil; Fischel-Ghodsian, Nathan; Rotter, Jerome I.; Pras, Mordechai; Shohat, Mordechai; Deaven, Larry L.; Gumucio, Deborah L.; Callen, David F.; Richards, Robert I.; Collins, Francis S.; Liu, P.Paul; Kastner, Daniel L.; Doggett, Norman A.
Abstract: In this paper we describe the assembly and restriction map of a 1.05-Mb cosmid contig spanning the candidate region for familial Mediterranean fever (FMF), a recessively inherited disorder of inflammation localized to 16p13.3. Using a combination of cosmid walking and screening for P1, PAC, BAC, and YAC clones, we have generated a contig of genomic clones spanning approximately 1050 kb that contains the FMF critical region. The map consists of 179 cosmid, 15 P1, 10 PAC, 3 BAC, and 17 YAC clones, anchored by 27 STS markers. Eight additional STSs have been developed from the approximately 700 kb immediately centromeric to this genomic region. Five of the 35 STSs are microsatellites that have not been previously reported. NotI and EcoRI mapping of the overlapping cosmids, hybridization of restriction fragments from cosmids to one another, and STS analyses have been used to validate the assembly of the contig. Our contig totally subsumes the 250-kb interval recently reported, by founder haplotype analysis, to contain the FMF gene. Thus, our high-resolution clone map provides an ideal resource for transcriptional mapping toward the eventual identification of this disease gene.
Keywords: Chromosomes, Artificial, Yeast
Chromosomes, Human, Pair 16
Humans
Familial Mediterranean Fever
DNA Primers
Genetic Markers
In Situ Hybridization, Fluorescence
Restriction Mapping
Cloning, Molecular
Base Sequence
Haplotypes
Cosmids
Sequence Tagged Sites
Molecular Sequence Data
DOI: 10.1006/geno.1997.4629
Published version: http://dx.doi.org/10.1006/geno.1997.4629
Appears in Collections:Aurora harvest 2
Genetics publications

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