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|Title:||Ribosomal RNA genes and the B chromosome of Brachycome dichromosomatica|
|Citation:||Heredity, 1995; 74(5):556-561|
|Publisher:||Oliver & Boyd|
|Tamzin M Donald, Carolyn R Leach, Angela Clough and Jeremy N Timmis|
|Abstract:||Fluorescence in situ hybridization (FISH) with biotinylated rDNA revealed the presence of an rRNA gene cluster on both the A and B chromosomes of Brachycome dichromosomatica, an Australian native ephemeral plant of the arid regions of south-eastern Australia. This species contains only two pairs of A chromosomes and up to three B chromosomes. The regular attachment of the B chromosome to a nucleolus suggests that these ribosomal RNA genes are transcribed. Southern hybridization of DNA from 0B and +B plants digested with a variety of restriction enzymes indicates that the rRNA genes on the A and B chromosomes are the same in sequence and methylation status.|
|Keywords:||Chromosomes; Plants; RNA, Plant; RNA, Ribosomal; Blotting, Southern; In Situ Hybridization, Fluorescence; Methylation|
|Appears in Collections:||Genetics publications|
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