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Type: Journal article
Title: Ribosomal RNA genes and the B chromosome of Brachycome dichromosomatica
Author: Donald, T.
Leach, C.
Clough, A.
Timmis, J.
Citation: Heredity, 1995; 74(5):556-561
Publisher: Oliver & Boyd
Issue Date: 1995
ISSN: 0018-067X
Statement of
Tamzin M Donald, Carolyn R Leach, Angela Clough and Jeremy N Timmis
Abstract: Fluorescence in situ hybridization (FISH) with biotinylated rDNA revealed the presence of an rRNA gene cluster on both the A and B chromosomes of Brachycome dichromosomatica, an Australian native ephemeral plant of the arid regions of south-eastern Australia. This species contains only two pairs of A chromosomes and up to three B chromosomes. The regular attachment of the B chromosome to a nucleolus suggests that these ribosomal RNA genes are transcribed. Southern hybridization of DNA from 0B and +B plants digested with a variety of restriction enzymes indicates that the rRNA genes on the A and B chromosomes are the same in sequence and methylation status.
Keywords: Chromosomes; Plants; RNA, Plant; RNA, Ribosomal; Blotting, Southern; In Situ Hybridization, Fluorescence; Methylation
RMID: 0030004296
DOI: 10.1038/hdy.1995.77
Appears in Collections:Genetics publications

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