Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/11518
Type: Journal article
Title: Novel Vibrio cholerae O139 genes involved in Lipopolysaccharide biosynthesis
Author: Stroeher, Uwe Horst
Parasivam, Gayathri
Dredge, Belinda Kate
Manning, Paul Alexander
Citation: Journal of Bacteriology, 1997; 179 (8):2740-2747
Issue Date: 1997
Statement of
Responsibility: 
Uwe H. Stroeher, Gayathri Parasivam, B. Kate Dredge, and Paul A. Manning
Abstract: The sequence of part of the rfb region of Vibrio cholerae serogroup O139 and the physical map of a 35-kb region of the O139 chromosome have been determined. The O139 rfb region presented contains a number of open reading frames which show similarities to other rfb and capsular biosynthesis genes found in members of the Enterobacteriaceae family and in V. cholerae O1. The cloned and sequenced region can complement the defects in O139 antigen biosynthesis in transposon insertions within the O139 rfb cluster. Linkage is demonstrated among IS1358 of V. cholerae O139, the rfb region, and the recently reported otnA and otnB genes (E. M. Bik, A. E. Bunschoten, R. D. Gouw, and F. R. Mooi, EMBO J. 14:209-216, 1995). In addition, the whole of this region has been linked to the rfaD gene. Furthermore, determination of the sequence flanking IS1358 has revealed homology to other rfb-like genes. The exact site of insertion with respect to rfaD is defined for the novel DNAs of both the Bengal and the Argentinian O139 isolates.
Published version: http://jb.asm.org/cgi/content/abstract/179/8/2740
Appears in Collections:Microbiology and Immunology publications

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