Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/115402
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Type: Journal article
Title: miR-542-3p prevents ovariectomy-induced osteoporosis in rats via targeting SFRP1
Author: Zhang, X.
Zhu, Y.
Zhang, C.
Liu, J.
Sun, T.
Li, D.
Na, Q.
Xian, C.
Wang, L.
Teng, Z.
Citation: Journal of Cellular Physiology, 2018; 233(9):6798-6806
Publisher: Wiley
Issue Date: 2018
ISSN: 0021-9541
1097-4652
Statement of
Responsibility: 
Xiguang Zhang, Yun Zhu, Chuanlin Zhang, Jianping Liu, Tianming Sun, Dan Li, Qiang Na, Cory J. Xian, Liping Wang, Zhaowei Teng
Abstract: Secreted frizzled-related protein-1 (SFRP1) is a negative regulatory molecule of the WNT signaling pathway and serves as a therapeutic target for bone formation in osteoporosis. In this study, we first established an ovariectomized (OVX) rat model to simulate postmenopausal osteoporosis and found significant changes in miR-542-3p and sFRP1 expression by RNA sequencing and qRT-PCR. In addition, there was a significant negative correlation between miR-542-3p and sFRP1 mRNA levels in postmenopausal women with osteoporosis. We found that miR-542-3p inhibited the expression of sFRP1 mRNA by luciferase reporter assay. When the miR-542-3p binding site in sFRP1 3'UTR was deleted, it did not affect its expression. Western blot results showed that miR-542-3p inhibited the expression of SFRP1 protein. The expression of SFRP1 was significantly increased in osteoblast-induced mesenchymal stem cells (MSC), whereas the expression of miR-542-3p was significantly decreased. And miR-542-3p transfected MSCs showed a significant increase in osteoblast-specific marker expression, indicating that miR-542-3p is necessary for MSC differentiation. Inhibition of miR-542-3p reduced bone formation, confirmed miR-542-3p play a role in bone formation in vivo. In general, these data suggest that miR-542-3p play an important role in bone formation via inhibiting SFRP1 expression and inducing osteoblast differentiation.
Keywords: differentiation; miRNA; osteoporosis; SFRP1
Rights: © 2018 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
RMID: 0030090120
DOI: 10.1002/jcp.26430
Grant ID: http://purl.org/au-research/grants/nhmrc/1094606
http://purl.org/au-research/grants/nhmrc/1042105
Appears in Collections:Paediatrics publications

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