Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/116059
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dc.contributor.authorLow, W.en
dc.contributor.authorNg, H.en
dc.contributor.authorMorton, C.en
dc.contributor.authorParker, M.en
dc.contributor.authorBatterham, P.en
dc.contributor.authorRobin, C.en
dc.date.issued2007en
dc.identifier.citationGenetics, 2007; 177(3):1363-1375en
dc.identifier.issn0016-6731en
dc.identifier.issn1943-2631en
dc.identifier.urihttp://hdl.handle.net/2440/116059-
dc.description.abstractAs classical phase II detoxification enzymes, glutathione S-transferases (GSTs) have been implicated in insecticide resistance and may have evolved in response to toxins in the niche-defining feeding substrates of Drosophila species. We have annotated the GST genes of the 12 Drosophila species with recently sequenced genomes and analyzed their molecular evolution. Gene copy number variation is attributable mainly to unequal crossing-over events in the large d and e clusters. Within these gene clusters there are also GST genes with slowly diverging orthologs. This implies that they have their own unique functions or have spatial/temporal expression patterns that impose significant selective constraints. Searches for positively selected sites within the GSTs identified G171K in GSTD1, a protein that has previously been shown to be capable of metabolizing the insecticide DDT. We find that the same radical substitution (G171K) in the substrate-binding domain has occurred at least three times in the Drosophila radiation. Homology-modeling places site 171 distant from the active site but adjacent to an alternative DDT-binding site. We propose that the parallel evolution observed at this site is an adaptive response to an environmental toxin and that sequencing of historical alleles suggests that this toxin was not a synthetic insecticide.en
dc.description.statementofresponsibilityWai Yee Low, Hooi Ling Ng, Craig J. Morton, Michael W. Parker, Philip Batterham and Charles Robinen
dc.language.isoenen
dc.publisherThe Genetics Society of Americaen
dc.rightsCopyright © 2007 by the Genetics Society of Americaen
dc.subjectAnimals; Drosophila; DDT; Glutathione Transferase; Drosophila Proteins; DNA Primers; Insecticides; Evolution, Molecular; Phylogeny; Species Specificity; Gene Duplication; Gene Deletion; Base Sequence; Insecticide Resistance; Genes, Insect; Multigene Family; Models, Genetic; Models, Molecular; Selection, Geneticen
dc.titleMolecular evolution of glutathione S-transferases in the genus Drosophilaen
dc.typeJournal articleen
dc.identifier.rmid0030075950en
dc.identifier.doi10.1534/genetics.107.075838en
dc.relation.granthttp://purl.org/au-research/grants/arc/DP0557497en
dc.identifier.pubid370483-
pubs.library.collectionAnimal and Veterinary Sciences publicationsen
pubs.library.teamDS06en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidLow, W. [0000-0002-0749-765X]en
Appears in Collections:Animal and Veterinary Sciences publications

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