Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/116640
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Type: Journal article
Title: Bacterial natural product biosynthetic domain composition in soil correlates with changes in latitude on a continent-wide scale
Author: Lemetre, C.
Maniko, J.
Charlop-Powers, Z.
Sparrow, B.
Lowe, A.
Brady, S.
Citation: Proceedings of the National Academy of Sciences of the United States of America, 2017; 114(44):11615-11620
Publisher: National Academy of Sciences
Issue Date: 2017
ISSN: 0027-8424
1091-6490
Statement of
Responsibility: 
Christophe Lemetrea, Jeffrey Manikoa, Zachary Charlop-Powersa, Ben Sparrow, Andrew J. Lowec and Sean F. Brady
Abstract: Although bacterial bioactive metabolites have been one of the most prolific sources of lead structures for the development of small-molecule therapeutics, very little is known about the environmental factors associated with changes in secondary metabolism across natural environments. Large-scale sequencing of environmental microbiomes has the potential to shed light on the richness of bacterial biosynthetic diversity hidden in the environment, how it varies from one environment to the next, and what environmental factors correlate with changes in biosynthetic diversity. In this study, the sequencing of PCR amplicons generated using primers targeting either ketosynthase domains from polyketide biosynthesis or adenylation domains from nonribosomal peptide biosynthesis was used to assess biosynthetic domain composition and richness in soils collected across the Australian continent. Using environmental variables collected at each soil site, we looked for environmental factors that correlated with either high overall domain richness or changes in the domain composition. Among the environmental variables we measured, changes in biosynthetic domain composition correlate most closely with changes in latitude and to a lesser extent changes in pH. Although it is unclear at this time the exact mix of factors that may drive the relationship between biosynthetic domain composition and latitude, from a practical perspective the identification of a latitudinal basis for differences in soil metagenome biosynthetic domain compositions should help guide future natural product discovery efforts.
Keywords: Chemical biogeography; eDNA; polyketide synthase; nonribosomal peptide synthetase; continental soil analysis
Rights: © 2017 National Academy of Sciences.
RMID: 0030077215
DOI: 10.1073/pnas.1710262114
Appears in Collections:Environment Institute publications

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