Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/118324
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | An optical fiber based immunosensor for localized detection of IL-1β in rat spinal cord |
Other Titles: | An optical fiber based immunosensor for localized detection of IL-1beta in rat spinal cord |
Author: | Zhang, K. Arman, A. Anwer, A. Hutchinson, M. Goldys, E. |
Citation: | Sensors and Actuators B: Chemical: international journal devoted to research and development of physical and chemical transducers, 2019; 282:122-129 |
Publisher: | Elsevier |
Issue Date: | 2019 |
ISSN: | 0925-4005 |
Statement of Responsibility: | Kaixin Zhang, Azim Arman, Ayad G. Anwer, Mark R. Hutchinson, Ewa M. Goldys |
Abstract: | Sensitive and localized measurements of cytokines is important in biomedicine as cytokines are produced locally where needed to induce an immune reaction. Here, we present a novel immunosensor deposited on the optical fiber surface. The sensor is capable of localized detection of interleukin-1beta (IL-1β) in the rat spinal cord. In this immunosensor, a stable immunocapture surface was formed via a biotin-streptavidin coupling strategy and fluorescent carboxylated supermagnetic iron oxide (SPIO)-IL-1β detection antibody conjugates were used for signal amplification. Under the optimal condition, the proposed immunosensor can be used for the estimation of IL-1β in vitro in the range from 3.13 pg.mL⁻¹ to 400 pg.mL⁻¹ with a detection limit of 1.12 pg.mL⁻¹. Furthermore, the performance of the fiber sensor was firstly assessed by ex-vivo monitoring the secretions of the rat macrophages stimulated by lipopolysaccharide (LPS), and the results demonstrated significant correlations with a commercial ELISA kit. Furthermore, the fiber sensor was successfully utilized to carry out a localized measurement of IL-1β in a spinal cord of an anesthetized rat. The result indicates that such fiber sensors can be used as an effective and sensitive tool for localized detection of IL-1β in vivo, in a range of research and clinical applications. |
Keywords: | Optical fiber; interleukin-1beta; in vivo; sensor; immunosensor |
Rights: | © 2018 Published by Elsevier B.V. |
DOI: | 10.1016/j.snb.2018.11.054 |
Grant ID: | http://purl.org/au-research/grants/arc/CE140100003 |
Published version: | http://dx.doi.org/10.1016/j.snb.2018.11.054 |
Appears in Collections: | Aurora harvest 4 Medicine publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.