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https://hdl.handle.net/2440/120869
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Type: | Journal article |
Title: | MALDI mass spectrometry imaging of early- and late-stage serous ovarian cancer tissue reveals stage-specific N-glycans |
Author: | Briggs, M.T. Condina, M.R. Ho, Y.Y. Everest-Dass, A.V. Mittal, P. Kaur, G. Oehler, M.K. Packer, N.H. Hoffmann, P. |
Citation: | Proteomics, 2019; 19(21-22):1800482-1-1800482-11 |
Publisher: | Wiley |
Issue Date: | 2019 |
ISSN: | 1615-9853 1615-9861 |
Statement of Responsibility: | Matthew T. Briggs, Mark R. Condina, Yin Ying Ho, Arun V. Everest-Dass, Parul Mittal, Gurjeet Kaur, Martin K. Oehler, Nicolle H. Packer, and Peter Hoffmann |
Abstract: | Epithelial ovarian cancer is one of the most fatal gynecological malignancies in adult women. As studies on protein N-glycosylation have extensively reported aberrant patterns in the ovarian cancer tumor microenvironment, obtaining spatial information will uncover tumor-specific N-glycan alterations in ovarian cancer development and progression. matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is employed to investigate N-glycan distribution on formalin-fixed paraffin-embedded ovarian cancer tissue sections from early- and late-stage patients. Tumor-specific N-glycans are identified and structurally characterized by porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry (PGC-LC-ESI-MS/MS), and then assigned to high-resolution images obtained from MALDI-MSI. Spatial distribution of 14 N-glycans is obtained by MALDI-MSI and 42 N-glycans (including structural and compositional isomers) identified and structurally characterized by LC-MS. The spatial distribution of oligomannose, complex neutral, bisecting, and sialylated N-glycan families are localized to the tumor regions of late-stage ovarian cancer patients relative to early-stage patients. Potential N-glycan diagnostic markers that emerge include the oligomannose structure, (Hex)₆ + (Man)₃ (GlcNAc)₂ , and the complex neutral structure, (Hex)₂ (HexNAc)₂ (Deoxyhexose)₁ + (Man)₃ (GlcNAc)₂. The distribution of these markers is evaluated using a tissue microarray of early- and late-stage patients. |
Keywords: | Formalin-fixed paraffin-embedded; mass spectrometry imaging; matrix-assisted laser desorption/ionization; N-glycan; ovarian cancer; tissue |
Rights: | © 2019 The Authors. Proteomics Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
DOI: | 10.1002/pmic.201800482 |
Grant ID: | http://purl.org/au-research/grants/arc/LP110100693 http://purl.org/au-research/grants/arc/CE140100003 |
Published version: | http://dx.doi.org/10.1002/pmic.201800482 |
Appears in Collections: | Aurora harvest 4 Medicine publications |
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