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https://hdl.handle.net/2440/12153
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Type: | Journal article |
Title: | Host defence peptides from the skin glands of the Australian Blue Mountains tree-frog Litoria citropa |
Author: | Wegener, K. Wabnitz, P. Carver, J. Bowie, J. Chia, B. Wallace, J. Tyler, M. |
Citation: | The Federation of European Biochemical Societies (FEBS) Journal, 1999; 265(2):627-637 |
Publisher: | WILEY-BLACKWELL |
Issue Date: | 1999 |
ISSN: | 0014-2956 1432-1033 |
Abstract: | Nineteen citropin peptides are present in the secretion from the granular dorsal glands of the Blue Mountains tree-frog Litoria citropa; 15 of these peptides are also present in the secretion from the submental gland. Two major peptides, citropin 1.1 (GLFDVIKKVASVIGGL-NH2), citropin 1.2 (GLFDIIKKVASVVGGL-NH2) and a minor peptide, citropin 1.3 (GLFDIIKKVASVIGGL-NH2) are wide-spectrum antibacterial peptides. The amphibian has an endoprotease which deactivates these membrane-active peptides by removing residues from the N-terminal end: loss of three residues gives the most abundant degradation products. The solution structure of the basic peptide citropin 1.1 has been determined by NMR spectroscopy [in a solvent mixture of trifluoroethanol/water (1 : 1)] to be an amphipathic alpha-helix with well-defined hydrophobic and hydrophilic regions. The additional four peptides produced by the dorsal glands are structurally related to the antibacterial citropin 1 peptides but contain three more residues at their C-terminus [e.g. citropin 1.1.3 (GLFDVIKKVASVIGLASP-OH)]. These peptides show minimal antibacterial activity; their role in the amphibian skin is not known. |
Keywords: | Skin Animals Anura Peptides Antimicrobial Cationic Peptides Proteins Amphibian Proteins Anti-Infective Agents Circular Dichroism Magnetic Resonance Spectroscopy Sequence Analysis Amino Acid Sequence Models, Molecular Molecular Sequence Data Mass Spectrometry |
DOI: | 10.1046/j.1432-1327.1999.00750.x |
Published version: | http://dx.doi.org/10.1046/j.1432-1327.1999.00750.x |
Appears in Collections: | Aurora harvest 7 Ecology, Evolution and Landscape Science publications |
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