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Type: Journal article
Title: Isolation of villous cytotrophoblasts from second trimester human placentas
Author: Jankovic-Karasoulos, T.
McAninch, D.
McCullough, D.
Wilson, R.L.
Bianco-Miotto, T.
Roberts, C.
Citation: Placenta, 2018; 74:55-58
Publisher: Elsevier
Issue Date: 2018
ISSN: 0143-4004
Statement of
Tanja Jankovic-Karasoulos, Dale McAninch, Dylan McCullough, Rebecca L.Wilson,Tina Bianco-Miotto, Claire T.Roberts
Abstract: Human placental functional studies are often performed using immortalised trophoblast cells and cell lines established from human choriocarcinomas, which, while practical, may not truly reflect trophoblast function in vivo. Primary trophoblast cultures derived from human placentas following pregnancy termination or delivery are more clinically relevant, but trophoblast isolation protocols are only available for 1st trimester and term placental tissues. Here we report a method for isolation and purification of primary villous cytotrophoblasts from 2nd trimester human placentas, that yields >99% trophoblast purity as shown by immunofluorescence.
Keywords: Placenta; Primary cell isolation; Second trimester; Trophoblasts
Rights: © 2018 Elsevier Ltd. All rights reserved.
RMID: 0030105650
DOI: 10.1016/j.placenta.2018.11.007
Appears in Collections:Obstetrics and Gynaecology publications

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