Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/121911
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Type: | Journal article |
Title: | Enzyme encapsulation in a porous hydrogen-bonded organic framework |
Author: | Liang, W. Carraro, F. Solomon, M.B. Bell, S.G. Amenitsch, H. Sumby, C.J. White, N.G. Falcaro, P. Doonan, C.J. |
Citation: | Journal of the American Chemical Society, 2019; 141(36):14298-14305 |
Publisher: | ACS Publications |
Issue Date: | 2019 |
ISSN: | 0002-7863 1520-5126 |
Statement of Responsibility: | Weibin Liang, Francesco Carraro, Marcello B. Solomon, Stephen G. Bell, Heinz Amenitsch, Christopher J. Sumby, Nicholas G. White, Paolo Falcaro, Christian J. Doonan |
Abstract: | Protection of biological assemblies is critical to applications in biotechnology, increasing the durability of enzymes in biocatalysis or potentially stabilizing biotherapeutics during transport and use. Here we show that a porous hydrogen-bonded organic framework (HOF) constructed from water-soluble tetra-amidinium (1·Cl4) and tetracarboxylate (2) building blocks can encapsulate and stabilize biomolecules to elevated temperature, proteolytic and denaturing agents, and extend the operable pH range for catalase activity. The HOF, which readily retains water within its framework structure, can also protect and retain the activity of enzymes such as alcohol oxidase, that are inactive when encapsulated within zeolitic imidazolate framework (ZIF) materials. Such HOF coatings could provide valid alternative materials to ZIFs: they are metal free, possess larger pore apertures, and are stable over a wider, more biologically relevant pH range. |
Keywords: | Zeolites Water Amides Carboxylic Acids Imidazoles Alcohol Oxidoreductases Molecular Conformation Hydrogen Bonding Hydrogen-Ion Concentration Particle Size Surface Properties Porosity |
Rights: | © 2019 American Chemical Society |
DOI: | 10.1021/jacs.9b06589 |
Published version: | http://dx.doi.org/10.1021/jacs.9b06589 |
Appears in Collections: | Aurora harvest 4 Chemistry and Physics publications |
Files in This Item:
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hdl_121911.pdf | Accepted version | 4.5 MB | Adobe PDF | View/Open |
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