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https://hdl.handle.net/2440/12334
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Type: | Journal article |
Title: | Differential tissue expression of epitopes of the tetraspanin CD151 recognised by monoclonal antibodies |
Author: | Geary, S. Cambareri, A. Sincock, P. Fitter, S. Ashman, L. |
Citation: | Tissue Antigens: immune response genetics, 2001; 58(3):141-153 |
Publisher: | Blackwell Munksgaard |
Issue Date: | 2001 |
ISSN: | 0001-2815 1399-0039 |
Abstract: | CD151, a member of the tetraspanin family of cell membrane proteins, is widely expressed in epithelial, endothelial and muscle cells as well as platelets and megakaryocytes. Several monoclonal antibodies recognising CD151 in transfected cells and immunoprecipitating typical bands of 28 and 32 kDa from cell lysates have been produced. Surprisingly, these antibodies show different patterns of staining on tissue sections and on haemopoietic cells. Here we show that these differences are at least in part due to masking of certain epitopes in integrin/CD151 complexes. These data have important implications for the use of monoclonal antibodies in studies of the distribution and function of CD151. Of six monoclonal antibodies from four laboratories, 11B1 was found to be the most reliable for detection of CD151 in different cell and tissue contexts. |
Keywords: | Muscles Epithelium Bone Marrow Cells Cells, Cultured Cell Line Animals Humans Mice Macromolecular Substances Membrane Proteins Integrins Integrin alpha3 Integrin alpha6 Antigens, CD Antibodies, Monoclonal Epitopes Fluorescent Antibody Technique Blotting, Western Immunoenzyme Techniques Precipitin Tests Transfection Antibody Specificity Tetraspanin 24 |
DOI: | 10.1034/j.1399-0039.2001.580301.x |
Published version: | http://dx.doi.org/10.1034/j.1399-0039.2001.580301.x |
Appears in Collections: | Aurora harvest 2 Ecology, Evolution and Landscape Science publications |
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