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Type: Journal article
Title: The spatio-temporal dynamics of mitochondrial membrane potential during oocyte maturation
Author: Al-Zubaidi, U.
Liu, J.
Cinar, O.
Robker, R.L.
Adhikari, D.
Carroll, J.
Citation: Molecular Human Reproduction, 2019; 25(11):697-705
Publisher: Oxford University Press
Issue Date: 2019
ISSN: 1360-9947
Statement of
Usama AL-Zubaidi, Jun Liu, Ozgur Cinar, Rebecca L Robker, Deepak Adhikari, and John Carroll
Abstract: Mitochondria are highly dynamic organelles and their distribution, structure and activity affect a wide range of cellular functions. Mitochondrial membrane potential (∆Ψm) is an indicator of mitochondrial activity and plays a major role in ATP production, redox balance, signaling and metabolism. Despite the absolute reliance of oocyte and early embryo development on mitochondrial function, there is little known about the spatial and temporal aspects of ΔΨm during oocyte maturation. The one exception is that previous findings using a ΔΨm indicator, JC-1, report that mitochondria in the cortex show a preferentially increased ΔΨm, relative to the rest of the cytoplasm. Using live-cell imaging and a new ratiometric approach for measuring ΔΨm in mouse oocytes, we find that ΔΨm increases through the time course of oocyte maturation and that mitochondria in the vicinity of the first meiotic spindle show an increase in ΔΨm, compared to other regions of the cytoplasm. We find no evidence for an elevated ΔΨm in the oocyte cortex. These findings suggest that mitochondrial activity is adaptive and responsive to the events of oocyte maturation at both a global and local level. In conclusion, we have provided a new approach to reliably measure ΔΨm that has shed new light onto the spatio-temporal regulation of mitochondrial function in oocytes and early embryos.
Keywords: Oocyte maturation; mitochrondria; membrane potential; JC-1; TMRM
Rights: © The Author(s) 2019. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact
RMID: 1000001385
DOI: 10.1093/molehr/gaz055
Appears in Collections:Obstetrics and Gynaecology publications

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