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https://hdl.handle.net/2440/123952
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Type: | Journal article |
Title: | Annexin-A1 deficiency exacerbates pathological remodelling of the mesenteric vasculature in insulin-resistant, but not insulin-deficient, mice |
Author: | Jelinic, M. Kahlberg, N. Leo, C.H. Ng, H.H. Rosli, S. Deo, M. Li, M. Finlayson, S. Walsh, J. Parry, L.J. Ritchie, R.H. Qin, C.X. |
Citation: | British Journal of Pharmacology, 2020; 177(7):1677-1691 |
Publisher: | Wiley |
Issue Date: | 2020 |
ISSN: | 0007-1188 1476-5381 |
Statement of Responsibility: | Maria Jelinic, Nicola Kahlberg, Chen Huei Leo, Hooi Hooi Ng, Sarah Rosli, Minh Deo, Mandy Li, Siobhan Finlayson, Jesse Walsh, Laura J. Parry, Rebecca H. Ritchie, Cheng Xue Qin |
Abstract: | Background and purpose: Arterial stiffness, a characteristic feature of diabetes, increases the risk of cardiovascular complications. Potential mechanisms that promote arterial stiffness in diabetes include oxidative stress, glycation and inflammation. The anti-inflammatory protein annexin-A1 has cardioprotective properties, particularly in the context of ischaemia. However, the role of endogenous annexin- A1 in the vasculature in both normal physiology and pathophysiology remains largely unknown. Hence, this study investigated the role of endogenous annexin-A1 in diabetes-induced remodelling of mouse mesenteric vasculature. Experimental approach: Insulin-resistance was induced in male mice (AnxA1+/+ and AnxA1-/-) with the combination of streptozotocin (55mg/kg i.p. x 3 days) with high fat diet (42% energy from fat) or citrate vehicle with normal chow diet (20-weeks). Insulin-deficiency was induced in a separate cohort of mice using a higher total streptozocin dose (55mg/kg i.p. x 5 days) on chow diet (16-weeks). At study endpoint, mesenteric artery passive mechanics were assessed by pressure myography. Key results: Insulin-resistance induced significant outward remodelling but had no impact on passive stiffness. Interestingly, vascular stiffness was significantly increased in AnxA1-/- mice when subjected to insulin-resistance. In contrast, insulindeficiency induced outward remodelling and increased volume compliance in mesenteric arteries, regardless of genotype. In addition, the annexin-A1 / formyl peptide receptor axis is upregulated in both insulin-resistant and insulin-deficient mice. Conclusion and implications: Our study provided the first evidence that endogenous AnxA1 may play an important vasoprotective role in the context of insulin-resistance. AnxA1-based therapies may provide additional benefits over traditional antiinflammatory strategies for reducing vascular injury in diabetes. |
Keywords: | Animals Mice Insulin Resistance Inflammation Insulin Annexin A1 Receptors, Formyl Peptide Male |
Rights: | © 2019 The British Pharmacological Society |
DOI: | 10.1111/bph.14927 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1081770 http://purl.org/au-research/grants/nhmrc/1059960 |
Published version: | http://dx.doi.org/10.1111/bph.14927 |
Appears in Collections: | Aurora harvest 4 Medicine publications |
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