Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Comparison of two transmission electron microscopy methods to visualize drug-induced alterations of gram-negative bacterial morphology
Author: Nguyen, H.T.
O’Donovan, L.
Venter, H.
Russell, C.C.
McCluskey, A.
Page, S.W.
Trott, D.J.
Ogunniyi, A.D.
Citation: Antibiotics, 2021; 10(3):307-307
Publisher: MDPI AG
Issue Date: 2021
ISSN: 2079-6382
Statement of
Hang Thi Nguyen, Lisa A. O’Donovan, Henrietta Venter, Cecilia C. Russell, Adam McCluskey, Stephen W. Page ... et al.
Abstract: In this study, we optimized and compared different transmission electron microscopy (TEM) methods to visualize changes to Gram-negative bacterial morphology induced by treatment with a robenidine analogue (NCL195) and colistin combination. Aldehyde-fixed bacterial cells (untreated, treated with colistin or NCL195 + colistin) were prepared using conventional TEM methods and compared with ultrathin Tokuyasu cryo-sections. The results of this study indicate superiority of ultrathin cryo-sections in visualizing the membrane ultrastructure of Escherichia coli and Pseudomonas aeruginosa, with a clear delineation of the outer and inner membrane as well as the peptidoglycan layer. We suggest that the use of ultrathin cryo-sectioning can be used to better visualize and understand drug interaction mechanisms on the bacterial cell membrane.
Keywords: Transmission electron microscopy; bacterial cell wall; bacterial membrane; Gram-negative bacteria; colistin; drug interaction; Tokuyasu cryo-ultramicrotomy
Rights: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// 4.0/).
DOI: 10.3390/antibiotics10030307
Grant ID:
Published version:
Appears in Collections:Aurora harvest 8
Pharmacology publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.