Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/131530
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Protein detection enabled using functionalised silk-binding peptides on a silk-coated optical fibre |
Author: | Capon, P.K. Horsfall, A.J. Li, J. Schartner, E.P. Khalid, A. Purdey, M.S. McLaughlin, R.A. Abell, A.D. |
Citation: | RSC Advances: an international journal to further the chemical sciences, 2021; 11(36):22334-22342 |
Publisher: | Royal Society of Chemistry |
Issue Date: | 2021 |
ISSN: | 2046-2069 2046-2069 |
Statement of Responsibility: | Patrick K. Capon, Aimee J. Horsfall, Jiawen Li, Erik P. Schartner, Asma Khalid, Malcolm S. Purdey ... et al. |
Abstract: | We present a new coating procedure to prepare optical fibre sensors suitable for use with protein analytes. We demonstrate this through the detection of AlexaFluor-532 tagged streptavidin by its binding to D-biotin that is functionalised onto an optical fibre, via incorporation in a silk fibroin fibre coating. The D-biotin was covalently attached to a silk-binding peptide to provide SBP–biotin, which adheres the D-biotin to the silk-coated fibre tip. These optical fibre probes were prepared by two methods. The first involves dip-coating the fibre tip into a mixture of silk fibroin and SBP–biotin, which distributes the SBP–biotin throughout the silk coating (method A). The second method uses two steps, where the fibre is first dip-coated in silk only, then SBP–biotin added in a second dip-coating step. This isolates SBP–biotin to the outer surface of the silk layer (method B). A series of fluorescence measurements revealed that only the surface bound SBP–biotin detects streptavidin with a detection limit of 15 μg mL−1. The fibre coatings are stable to repeated washing and long-term exposure to water. Formation of silk coatings on fibres using commercial aqueous silk fibroin was found to be inhibited by a lithium concentration of 200 ppm, as determined by atomic absorption spectroscopy. This was reduced to less than 20 ppm by dialysis against water, and was found to successfully form a coating on optical fibres. |
Rights: | This journal is © The Royal Society of Chemistry 2021. |
DOI: | 10.1039/d1ra03584c |
Grant ID: | http://purl.org/au-research/grants/arc/CE140100003 http://purl.org/au-research/grants/nhmrc/2001646 http://purl.org/au-research/grants/nhmrc/102093 http://purl.org/au-research/grants/arc/LP150100657 http://purl.org/au-research/grants/nhmrc/1178912 http://purl.org/au-research/grants/nhmrc/2002254 |
Published version: | http://dx.doi.org/10.1039/d1ra03584c |
Appears in Collections: | Aurora harvest 4 Chemistry publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.