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Type: Journal article
Title: PCR differentiation of commercial yeast strains using intron splice site primers
Author: de Barros Lopes, Miguel
Soden, Alison
Henschke, Paul A.
Langridge, Peter
Citation: Applied and Environmental Microbiology, 1996; 62(12):4514-4520
Publisher: American Society for Microbiology
Issue Date: 1996
ISSN: 0099-2240
Statement of
Miguel de Barros Lopes, Alison Soden, Paul A. Henschke, and Peter Langridge
Abstract: The increased use of pure starter cultures in the wine industry has made it necessary to develop a rapid and simple identification system for yeast strains. A method based upon the PCR using oligonucleotide primers that are complementary to intron splice sites has been developed. Since most introns are not essential for gene function, introns have evolved with minimal constraint. By targeting these highly variable sequences, the PCR has proved to be very effective in uncovering polymorphisms in commercial yeast strains. The speed of the method and the ability to analyze many samples in a single day permit the monitoring of specific yeast strains during fermentations. Furthermore, the simplicity of the technique, which does not require the isolation of DNA, makes it accessible to industrial laboratories that have limited molecular expertise and resources.
Rights: Copyright © 1996, American Society for Microbiology
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Appears in Collections:Agriculture, Food and Wine publications

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