Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/13319
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dc.contributor.authorde Barros Lopes, Miguelen
dc.contributor.authorSoden, Alisonen
dc.contributor.authorHenschke, Paul A.en
dc.contributor.authorLangridge, Peteren
dc.date.issued1996en
dc.identifier.citationApplied and Environmental Microbiology, 1996; 62(12):4514-4520en
dc.identifier.issn0099-2240en
dc.identifier.urihttp://hdl.handle.net/2440/13319-
dc.description.abstractThe increased use of pure starter cultures in the wine industry has made it necessary to develop a rapid and simple identification system for yeast strains. A method based upon the PCR using oligonucleotide primers that are complementary to intron splice sites has been developed. Since most introns are not essential for gene function, introns have evolved with minimal constraint. By targeting these highly variable sequences, the PCR has proved to be very effective in uncovering polymorphisms in commercial yeast strains. The speed of the method and the ability to analyze many samples in a single day permit the monitoring of specific yeast strains during fermentations. Furthermore, the simplicity of the technique, which does not require the isolation of DNA, makes it accessible to industrial laboratories that have limited molecular expertise and resources.en
dc.description.statementofresponsibilityMiguel de Barros Lopes, Alison Soden, Paul A. Henschke, and Peter Langridgeen
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.rightsCopyright © 1996, American Society for Microbiologyen
dc.source.urihttp://aem.asm.org/content/62/12/4514.abstracten
dc.titlePCR differentiation of commercial yeast strains using intron splice site primersen
dc.typeJournal articleen
Appears in Collections:Agriculture, Food and Wine publications

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