Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/134136
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Pioglitazone rapidly reduces neuropathic pain through astrocyte and nongenomic PPARγ mechanisms |
Other Titles: | Pioglitazone rapidly reduces neuropathic pain through astrocyte and nongenomic PPARgamma mechanisms |
Author: | Griggs, R.B. Donahue, R.R. Morgenweck, J. Grace, P.M. Sutton, A. Watkins, L.R. Taylor, B.K. |
Citation: | Pain, 2015; 156(3):469-482 |
Publisher: | International Association for the Study of Pain; Ovid |
Issue Date: | 2015 |
ISSN: | 0304-3959 1872-6623 |
Statement of Responsibility: | Ryan B. Griggs, Renee R. Donahue, Jenny Morgenweck, Peter M. Grace, Amanda Sutton, Linda R. Watkins, Bradley K. Taylor |
Abstract: | Repeated administration of peroxisome proliferator-activated receptor gamma (PPARγ) agonists reduces neuropathic pain-like behavior and associated changes in glial activation in the spinal cord dorsal horn. As PPARγ is a nuclear receptor, sustained changes in gene expression are widely believed to be the mechanism of pain reduction. However, we recently reported that a single intrathecal (i.t.) injection of pioglitazone, a PPARγ agonist, reduced hyperalgesia within 30 minutes, a time frame that is typically less than that required for genomic mechanisms. To determine the very rapid antihyperalgesic actions of PPARγ activation, we administered pioglitazone to rats with spared nerve injury and evaluated hyperalgesia. Pioglitazone inhibited hyperalgesia within 5 minutes of injection, consistent with a nongenomic mechanism. Systemic or i.t. administration of GW9662, a PPARγ antagonist, inhibited the antihyperalgesic actions of intraperitoneal or i.t. pioglitazone, suggesting a spinal PPARγ-dependent mechanism. To further address the contribution of nongenomic mechanisms, we blocked new protein synthesis in the spinal cord with anisomycin. When coadministered intrathecally, anisomycin did not change pioglitazone antihyperalgesia at an early 7.5-minute time point, further supporting a rapid nongenomic mechanism. At later time points, anisomycin reduced pioglitazone antihyperalgesia, suggesting delayed recruitment of genomic mechanisms. Pioglitazone reduction of spared nerve injury-induced increases in GFAP expression occurred more rapidly than expected, within 60 minutes. We are the first to show that activation of spinal PPARγ rapidly reduces neuropathic pain independent of canonical genomic activity. We conclude that acute pioglitazone inhibits neuropathic pain in part by reducing astrocyte activation and through both genomic and nongenomic PPARγ mechanisms. |
Keywords: | Neuropathic; pain; PPAR gamma; astrocyte; pioglitazone; nongenomic |
Rights: | © 2015 the International Association for the Study of Pain |
DOI: | 10.1097/01.j.pain.0000460333.79127.be |
Grant ID: | 5R01 N5062306 R01NS045954 T32NS077889 F31NS083292 http://purl.org/au-research/grants/nhmrc/1054091 |
Published version: | http://dx.doi.org/10.1097/01.j.pain.0000460333.79127.be |
Appears in Collections: | Pharmacology publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.