Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/134484
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Type: | Journal article |
Title: | Spiked nanostructures disrupt fungal biofilm and impart increased sensitivity to antifungal treatment |
Author: | Hayles, A. Bright, R. Wood, J. Palms, D. Zilm, P. Brown, T. Barker, D. Vasilev, K. |
Citation: | Advanced Materials Interfaces, 2022; 9(12):2102353-1-2102353-13 |
Publisher: | Wiley |
Issue Date: | 2022 |
ISSN: | 2196-7350 2196-7350 |
Statement of Responsibility: | Andrew Hayles, Richard Bright, Jonathan Wood, Dennis Palms, Peter Zilm, Toby Brown, Dan Barker, and Krasimir Vasilev |
Abstract: | There is a globally increasing demand for medically implanted devices, partly spurred by an aging population. In parallel, there is a proportionate increase in implant associated infection. Much focus has been directed toward the development of techniques to fabricate nanostructured antimicrobial biomaterials to mitigate infection. The present study investigates the interaction of the fungal pathogen Candida albicans with an antimicrobial surface bearing nanoscale protrusions. C. albicans cells were observed to be affected by cell wall stress, which impeded its ability to switch to a hyphal phenotype. There are significant differences in the expression of C. albicans virulence-associated genes between the untreated and nanostructured surfaces. To determine whether the observed inhibition of C. albicans would also sensitize it to antifungal drugs, a culture is established for 3 days on the nanostructured surface before being treated with the antifungal drug amphotericin B. The drug was able to kill all cells on the nanostructured surface at sub-clinical concentrations, while remaining ineffective against cultures grown on a smooth control surface. These findings may eventually prove to be impactful in the clinic, as clinicians may be able to reduce antifungal drug dosages and minimize the effects of drug associated toxicity. |
Keywords: | antifungal drugs; hydrothermal etching; implants; mechano-bactericidal effect; morphogenesis |
Description: | First published: February 4, 2022 |
Rights: | © 2022 The Authors. Advanced Materials Interfaces published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
DOI: | 10.1002/admi.202102353 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1194466 |
Published version: | http://dx.doi.org/10.1002/admi.202102353 |
Appears in Collections: | Dentistry publications |
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hdl_134484.pdf | Published version | 3.81 MB | Adobe PDF | View/Open |
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