Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/13477
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Type: Journal article
Title: Cloning plant genes differentially expressed during colonization of roots of Hordeum vulgare by the vesicular-arbuscular mycorrhizal fungus Glomus intraradices
Author: Murphy, P.
Langridge, P.
Smith, S.
Citation: New Phytologist, 1997; 135(2):291-301
Publisher: CAMBRIDGE UNIV PRESS
Issue Date: 1997
ISSN: 0028-646X
1469-8137
Statement of
Responsibility: 
P. J. Murphy, P. Langridge and S. E. Smith
Abstract: A model system was developed for the molecular analysis of plant-mycorrhizal fungus interactions using Hordeum vulgare cv. Galleon and Glomus intraradices Schenk and Smith. High levels of early-colonization stage mycorrhizas (20–35 % of the total root length) were routinely obtained in the roots of 12–14–d-old barley seedlings. Detailed colonization studies showed that most colonization occurred in the first 5–10 lateral roots, and that colonization in primary roots and younger lateral roots was low. Differential screening of a cDNA library prepared from the highly colonized region of the root system and subsequent northern analyses identified four differentially expressed cDNA clones. Two clones, BMR6 and BMR78, detect RNA transcripts that accumulate to much higher levels in mycorrhizal roots than in non-mycorrhizal root tissues. DNA hybridization analysis confirmed that both of the clones were of plant rather than fungal origin. Hybridization and sequence analysis strongly suggests that BMR78 represents a partial cDNA of a low copy proton-ATPase gene. This finding is consistent with the extensive proliferation of the periarbuscular membrane and high ATPase activity associated with it. BM6 detects a low copy gene whose function is unknown.
Keywords: ATPases; Colonization; Gene expression; VA mycorrhiza
Rights: Copyright status unknown
RMID: 0030003694
DOI: 10.1046/j.1469-8137.1997.00652.x
Appears in Collections:Agriculture, Food and Wine publications

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