Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/13479
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Regulation of genes encoding beta-D-glucan glucohydrolases in barley (Hordeum vulgare)
Author: Harvey, A.
Hrmova, M.
Fincher, G.
Citation: Physiologia Plantarum, 2001; 113(1):108-120
Publisher: Blackwell Munksgaard
Issue Date: 2001
ISSN: 0031-9317
1399-3054
Statement of
Responsibility: 
Andrew J. Harvey, Maria Hrmova, Geoffrey B. Fincher
Abstract: Expression patterns of barley β-D-glucan glucohydrolase genes were monitored using cDNAs encoding isoenzymes ExoI and ExoII. The cDNAs were isolated from 5-day-old seedling libraries. The enzymes are encoded by a small gene family, in which marked differences in codon usage are evident. The cDNAs can be used as specific probes for two subfamilies of β-D-glucan glucohydrolase genes. Genes of both subfamilies are transcribed in the scutellum of germinated grain, in elongating coleoptiles, and in young roots and leaves. Low levels of mRNA for the isoenzyme ExoI gene subfamily could be detected in aleurone layers of germinated grain. Most of the β-D-glucan glucohydrolase activity can be extracted from tissues with dilute aqueous buffers. Enzyme activity is highest in young leaves and elongating coleoptiles, but is not well-correlated with mRNA levels. The expression patterns are consistent with proposed roles for β-glucan glucohydrolases in the turnover or modification of cell-wall (1→3,1→4)-β-D-glucans in elongating coleoptiles and in young vegetative tissues.
Description: The definitive version is available at www.blackwell-synergy.com
RMID: 0020010125
DOI: 10.1034/j.1399-3054.2001.1130115.x
Appears in Collections:Agriculture, Food and Wine publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.