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https://hdl.handle.net/2440/13577
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Type: | Journal article |
Title: | Development of YLM, a codominant PCR marker closely linked to the Yd2 gene for resistance to barley yellow dwarf disease |
Author: | Paltridge, N. Collins, N. Bendahmane, A. Symons, R. |
Citation: | Theoretical and Applied Genetics: international journal of plant breeding research, 1998; 96(8):1170-1177 |
Publisher: | SPRINGER |
Issue Date: | 1998 |
ISSN: | 0040-5752 1432-2242 |
Statement of Responsibility: | Paltridge, N. G. ; Collins, N. C. ; Bendahmane, A. ; Symons, R. H. |
Abstract: | The Yd2 gene in barley provides protection against barley yellow dwarf luteovirus (BYDV), the most economically devastating virus of cereals worldwide. Because resistance assays to identify Yd2-containing individuals from breeding populations are often difficult, we have developed a closely linked, codominant PCR-based marker for Yd2 using AFLP marker technology. The marker, designated YLM, can be amplified from barley genomic DNA prepared using a rapid and simple extraction procedure and, in a survey of more than 100 barley genotypes, was found to be polymorphic between most Yd2 and non-Yd2 lines. The YLM therefore shows excellent potential as a tool for selecting Yd2-carrying segregants in barley breeding programmes. |
DOI: | 10.1007/s001220050853 |
Published version: | http://dx.doi.org/10.1007/s001220050853 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 7 |
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