Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||Reduced Surfactant Contributes to Increased Lung Stiffness Induced by Rapid Inspiratory Flow|
|Citation:||Lung: an international journal on lungs, airways and breathing, 2020; 198(1):43-52|
|Publisher:||Springer Science and Business Media LLC|
|Andrew D. Bersten, Malgorzata Krupa, Kim Griggs, Dani, Louise Dixon|
|Abstract:||Introduction The mechanism of fast inspiratory flow rate (VI′) induced lung injury is unclear. As fast VI′ increases hysteresis, a measure of surface tension at the air–liquid interface, surfactant release or function may be important. This experimental study examines the contribution of impaired surfactant release or function to dynamic-VILI. Methods Isolated perfused lungs from male Sprague Dawley rats were randomly allocated to four groups: a long or short inspiratory time (Ti = 0.5 s; slow VI′ or Ti = 0.1 s; fast VI′) at PEEP of 2 or 10 cmH₂O. Tidal volume was constant (7 ml/kg), with f = 60 breath/min. Forced impedance mechanics (tissue elastance (Htis), tissue resistance (Gtis) and airway resistance (Raw) were measured at 30, 60 and 90 min following which the lung was lavaged for surfactant phospholipids (PL) and disaturated PL (DSP). Results Fast VI′ resulted in a stiffer lung. Concurrently, PL and DSP were decreased in both tubular myelin rich and poor fractions. Phospholipid decreases were similar with PEEP. In a subsequent cohort, laser confocal microscopy-based assessment demonstrated increased cellular injury with increased VI′ at both 30 and 90 min ventilation. Conclusion Rapid VI′ may contribute to ventilator induced lung injury (VILI) through reduced surfactant release and/or more rapid reuptake despite unchanged tidal stretch.|
|Keywords:||Lung mechanics; Ventilator induced lung injury; Cytokines; Inspiratory flow rate; Surfactant; Confocal microscopy|
|Rights:||© Springer Science+Business Media, LLC, part of Springer Nature 2020|
|Appears in Collections:||Physics publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.