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Type: Journal article
Title: Reduced Surfactant Contributes to Increased Lung Stiffness Induced by Rapid Inspiratory Flow
Author: Bersten, A.D.
Krupa, M.
Griggs, K.
Dixon, D.L.
Citation: Lung: an international journal on lungs, airways and breathing, 2020; 198(1):43-52
Publisher: Springer Science and Business Media LLC
Issue Date: 2020
ISSN: 0341-2040
Statement of
Andrew D. Bersten, Malgorzata Krupa, Kim Griggs, Dani, Louise Dixon
Abstract: Introduction The mechanism of fast inspiratory flow rate (VI′) induced lung injury is unclear. As fast VI′ increases hysteresis, a measure of surface tension at the air–liquid interface, surfactant release or function may be important. This experimental study examines the contribution of impaired surfactant release or function to dynamic-VILI. Methods Isolated perfused lungs from male Sprague Dawley rats were randomly allocated to four groups: a long or short inspiratory time (Ti = 0.5 s; slow VI′ or Ti = 0.1 s; fast VI′) at PEEP of 2 or 10 cmH₂O. Tidal volume was constant (7 ml/kg), with f = 60 breath/min. Forced impedance mechanics (tissue elastance (Htis), tissue resistance (Gtis) and airway resistance (Raw) were measured at 30, 60 and 90 min following which the lung was lavaged for surfactant phospholipids (PL) and disaturated PL (DSP). Results Fast VI′ resulted in a stiffer lung. Concurrently, PL and DSP were decreased in both tubular myelin rich and poor fractions. Phospholipid decreases were similar with PEEP. In a subsequent cohort, laser confocal microscopy-based assessment demonstrated increased cellular injury with increased VI′ at both 30 and 90 min ventilation. Conclusion Rapid VI′ may contribute to ventilator induced lung injury (VILI) through reduced surfactant release and/or more rapid reuptake despite unchanged tidal stretch.
Keywords: Lung mechanics; Ventilator induced lung injury; Cytokines; Inspiratory flow rate; Surfactant; Confocal microscopy
Rights: © Springer Science+Business Media, LLC, part of Springer Nature 2020
DOI: 10.1007/s00408-019-00317-1
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Appears in Collections:Physics publications

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