Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/139380
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Type: | Journal article |
Title: | An accurate and cost-effective alternative method for measuring cell migration with the circular wound closure assay |
Author: | De Ieso, M.L. Pei, J.V. |
Citation: | Bioscience Reports: molecular and cellular biology of the cell surface, 2018; 38(5):1-9 |
Publisher: | Portland Press |
Issue Date: | 2018 |
ISSN: | 0144-8463 1573-4935 |
Statement of Responsibility: | Michael L. De Ieso and Jinxin Victor Pei |
Abstract: | Cell migration is important in many physiological and pathological processes. Mechanisms of two-dimensional cell migration have been investigated most commonly by evaluating rates of cell migration into linearly scratched zones on the surfaces of culture plates. Here, we present a detailed description of a simple adaptation for the well-known and popular wound closure assay, using a circular wound instead of a straight line. This method demonstrates improved precision, reproducibility, and sampling objectivity for measurements of wound sizes as compared with classic scratch assays, enabling more accurate calculations of migration rate. The added benefits of the method are simplicity and low cost as compared with commercially available assays for generating circular wounds. |
Keywords: | cell migration; circular wound closure; scratch assay |
Rights: | © 2018 The Author(s). This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY). |
DOI: | 10.1042/BSR20180698 |
Grant ID: | http://purl.org/au-research/grants/arc/DP160104641 |
Published version: | http://dx.doi.org/10.1042/bsr20180698 |
Appears in Collections: | Medicine publications |
Files in This Item:
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hdl_139380.pdf | Published version | 1.72 MB | Adobe PDF | View/Open |
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