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|Title:||Biosynthesis, characterisation and direct high-performance liquid chromatographic analysis of gemfibrozil 1-O-β-acylglucuronide|
|Other Titles:||Biosynthesis, characterisation and direct high-performance liquid chromatographic analysis of gemfibrozil 1-O-beta-acylglucuronide|
|Citation:||Journal of Chromatography, 1995; 665(2):345-353|
|Benedetta C. Sallustio, Barbara A. Fairchild|
|Abstract:||Gemfibrozil 1-O-β-acylglucuronide was purified from the urine of a volunteer administered gemfibrozil, and an isocratic reversed-phase HPLC method was developed for its direct measurement. Quantitation of gemfibrozil and gemfibrozil 1-O-β-acylglucuronide was carried out from plasma, following extraction from acidified specimens into ethyl acetate, on a 5-μm CN reversed-phase column with a mobile phase (pH 3.5) containing acetonitrile, tetrabutylammonium sulphate and distilled water, using fluorescence detection at 284 nm excitation and 316 nm emission. Calibration curves were linear for both compounds over a concentration range of 0.1 to 40 mg/l, with intra-assay coefficients of variation <5% at concentrations of 20.0, 2.0 and 0.2 mg/l, and inter-assay coefficients of variation <10%. No degradation of gemfibrozil 1-O-β-acylglucuronide was detected as a result of the analytical procedure. However, a preliminary application of the method indicates that gemfibrozil acylglucuronide is chemically unstable undergoing intra-molecular rearrangement and hydrolysis under physiological conditions.|
|Keywords:||Humans; Gemfibrozil; Glucuronates; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Magnetic Resonance Spectroscopy; Drug Stability; Hydrogen-Ion Concentration; Quality Control; Mass Spectrometry|
|Rights:||Copyright © 1995 Published by Elsevier B.V.|
|Appears in Collections:||Pharmacology publications|
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