Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/14463
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Type: Journal article
Title: Quantification of flunitrazepam's oxidative metabolites, 3-hydroxyflunitrazepam and desmethylflunitrazepam, in hepatic microsomal incubations by high performance liquid chromatography.
Author: Coller, J.
Somogyi, A.
Bochner, F.
Citation: Journal of Chromatography B.., 1998; 719(1-2):87-92
Publisher: ELSEVIER SCIENCE BV
Issue Date: 1998
ISSN: 0378-4347
1878-5603
Abstract: A high-performance liquid chromatographic assay for the quantification of the oxidative metabolites of flunitrazepam, 3-hydroxyflunitrazepam and desmethylflunitrazepam, in human liver microsomal incubations was developed. Both metabolites were quantifiable in a single assay following a solvent extraction and reversed-phase high-performance liquid chromatography with UV detection. Standard curve concentrations for both metabolites ranged from 0.2 to 10 microM. Assay performance was determined using quality control samples and the intra- and inter-day accuracy and precision as determined by the coefficient of variations which were less than 15% (0.5-6 microM) for both metabolites. This method provides good precision and accuracy for use in kinetic studies of the oxidative metabolism of flunitrazepam in human liver microsomes.
Keywords: Microsomes, Liver; Humans; Flunitrazepam; Anti-Anxiety Agents; Calibration; Chromatography, High Pressure Liquid; Spectrophotometry, Ultraviolet; Reproducibility of Results; Reference Standards
RMID: 0030003398
DOI: 10.1016/S0378-4347(98)00383-1
Appears in Collections:Pharmacology publications

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