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Type: Journal article
Title: Quantification of flunitrazepam's oxidative metabolites, 3-hydroxyflunitrazepam and desmethylflunitrazepam, in hepatic microsomal incubations by high performance liquid chromatography.
Author: Coller, J.
Somogyi, A.
Bochner, F.
Citation: Journal of Chromatography - Biomedical Applications, 1998; 719(1-2):87-92
Issue Date: 1998
ISSN: 0378-4347
Abstract: A high-performance liquid chromatographic assay for the quantification of the oxidative metabolites of flunitrazepam, 3-hydroxyflunitrazepam and desmethylflunitrazepam, in human liver microsomal incubations was developed. Both metabolites were quantifiable in a single assay following a solvent extraction and reversed-phase high-performance liquid chromatography with UV detection. Standard curve concentrations for both metabolites ranged from 0.2 to 10 microM. Assay performance was determined using quality control samples and the intra- and inter-day accuracy and precision as determined by the coefficient of variations which were less than 15% (0.5-6 microM) for both metabolites. This method provides good precision and accuracy for use in kinetic studies of the oxidative metabolism of flunitrazepam in human liver microsomes.
Keywords: Microsomes, Liver
Anti-Anxiety Agents
Chromatography, High Pressure Liquid
Spectrophotometry, Ultraviolet
Reproducibility of Results
Reference Standards
DOI: 10.1016/S0378-4347(98)00383-1
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Appears in Collections:Aurora harvest 7
Pharmacology publications

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