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|Title:||Determination of perhexiline and hydroxyperhexiline in plasma by liquid chromatography-mass spectrometry|
|Citation:||Journal of Chromatography. B, Analytical Techniques in the Biomedical and Life Sciences, 2004; 805(1):87-91|
|Publisher:||Elsevier Science BV|
|Abstract:||A method for the quantitative determination of perhexiline and its main hydroxylated metabolites in human plasma, based on liquid chromatography-mass spectrometry (LC-MS), was developed. The method used protein precipitation with acetonitrile followed by dilution with water and subsequent direct injection of the extract into the LC-MS system. Hexadiline was used as internal standard and the intra-assay coefficients of variation were <or=5% for perhexiline and cis-hydroxyperhexiline over the target concentration range in patients. The lower limits of quantification were 0.005mg/l for perhexiline and 0.015mg/l for cis-hydroxyperhexiline, and the measuring ranges were from 0.05 to 3.0 and from 0.2 to 6.0mg/l, respectively. The method was compared with an established HPLC method with fluorescence detection and the correlation between the methods was close to 1 for both compounds. The predominant form of hydroxyperhexiline in 87% of the patient samples was found to be one of the diastereomeric pairs of cis-hydroxyperhexiline. In patients not forming this metabolite, trans-hydroxyperhexiline could be detected. We conclude that the present LC-MS method is suitable for use in a clinical routine laboratory.|
|Keywords:||Humans; Perhexiline; Cardiovascular Agents; Chromatography, High Pressure Liquid; Isomerism; Reference Standards; Mass Spectrometry|
|Appears in Collections:||Pharmacology publications|
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