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https://hdl.handle.net/2440/14466
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DC Field | Value | Language |
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dc.contributor.author | Beck, O. | - |
dc.contributor.author | Stephanson, N. | - |
dc.contributor.author | Morris, R. | - |
dc.contributor.author | Sallustio, B. | - |
dc.contributor.author | Hjemdahl, P. | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2004; 805(1):87-91 | - |
dc.identifier.issn | 1570-0232 | - |
dc.identifier.issn | 1873-376X | - |
dc.identifier.uri | http://hdl.handle.net/2440/14466 | - |
dc.description.abstract | A method for the quantitative determination of perhexiline and its main hydroxylated metabolites in human plasma, based on liquid chromatography-mass spectrometry (LC-MS), was developed. The method used protein precipitation with acetonitrile followed by dilution with water and subsequent direct injection of the extract into the LC-MS system. Hexadiline was used as internal standard and the intra-assay coefficients of variation were <or=5% for perhexiline and cis-hydroxyperhexiline over the target concentration range in patients. The lower limits of quantification were 0.005mg/l for perhexiline and 0.015mg/l for cis-hydroxyperhexiline, and the measuring ranges were from 0.05 to 3.0 and from 0.2 to 6.0mg/l, respectively. The method was compared with an established HPLC method with fluorescence detection and the correlation between the methods was close to 1 for both compounds. The predominant form of hydroxyperhexiline in 87% of the patient samples was found to be one of the diastereomeric pairs of cis-hydroxyperhexiline. In patients not forming this metabolite, trans-hydroxyperhexiline could be detected. We conclude that the present LC-MS method is suitable for use in a clinical routine laboratory. | - |
dc.language.iso | en | - |
dc.publisher | Elsevier Science BV | - |
dc.source.uri | http://dx.doi.org/10.1016/j.jchromb.2004.02.019 | - |
dc.subject | Humans | - |
dc.subject | Perhexiline | - |
dc.subject | Cardiovascular Agents | - |
dc.subject | Chromatography, High Pressure Liquid | - |
dc.subject | Isomerism | - |
dc.subject | Reference Standards | - |
dc.subject | Mass Spectrometry | - |
dc.title | Determination of perhexiline and hydroxyperhexiline in plasma by liquid chromatography-mass spectrometry | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.jchromb.2004.02.019 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Sallustio, B. [0000-0002-0186-3073] | - |
Appears in Collections: | Aurora harvest 7 Pharmacology publications |
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