Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/14466
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dc.contributor.authorBeck, O.en
dc.contributor.authorStephanson, N.en
dc.contributor.authorMorris, R.en
dc.contributor.authorSallustio, B.en
dc.contributor.authorHjemdahl, P.en
dc.date.issued2004en
dc.identifier.citationJournal of Chromatography. B, Analytical Techniques in the Biomedical and Life Sciences, 2004; 805(1):87-91en
dc.identifier.issn1570-0232en
dc.identifier.issn1873-376Xen
dc.identifier.urihttp://hdl.handle.net/2440/14466-
dc.description.abstractA method for the quantitative determination of perhexiline and its main hydroxylated metabolites in human plasma, based on liquid chromatography-mass spectrometry (LC-MS), was developed. The method used protein precipitation with acetonitrile followed by dilution with water and subsequent direct injection of the extract into the LC-MS system. Hexadiline was used as internal standard and the intra-assay coefficients of variation were <or=5% for perhexiline and cis-hydroxyperhexiline over the target concentration range in patients. The lower limits of quantification were 0.005mg/l for perhexiline and 0.015mg/l for cis-hydroxyperhexiline, and the measuring ranges were from 0.05 to 3.0 and from 0.2 to 6.0mg/l, respectively. The method was compared with an established HPLC method with fluorescence detection and the correlation between the methods was close to 1 for both compounds. The predominant form of hydroxyperhexiline in 87% of the patient samples was found to be one of the diastereomeric pairs of cis-hydroxyperhexiline. In patients not forming this metabolite, trans-hydroxyperhexiline could be detected. We conclude that the present LC-MS method is suitable for use in a clinical routine laboratory.en
dc.language.isoenen
dc.publisherElsevier Science BVen
dc.subjectHumans; Perhexiline; Cardiovascular Agents; Chromatography, High Pressure Liquid; Isomerism; Reference Standards; Mass Spectrometryen
dc.titleDetermination of perhexiline and hydroxyperhexiline in plasma by liquid chromatography-mass spectrometryen
dc.typeJournal articleen
dc.identifier.rmid0020040415en
dc.identifier.doi10.1016/j.jchromb.2004.02.019en
dc.identifier.pubid57050-
pubs.library.collectionPharmacology publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidSallustio, B. [0000-0002-0186-3073]en
Appears in Collections:Pharmacology publications

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