Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/14586
Citations
Scopus Web of ScienceĀ® Altmetric
?
?
Type: Journal article
Title: Cylindrospermopsin occurrence in two German lakes and preliminary assessment of toxicity and toxin production of Cylindrospermopsis raciborskii (Cyanobacteria) isolates
Author: Fastner, J.
Heinze, R.
Humpage, A.
Mischke, U.
Eaglesham, G.
Chorus, I.
Citation: Toxicon, 2003; 42(3):313-321
Publisher: Pergamon-Elsevier Science Ltd
Issue Date: 2003
ISSN: 0041-0101
1879-3150
Abstract: Cylindrospermopsis raciborskii, a freshwater cyanobacterium of tropical origin, is not only increasingly found in (sub) tropical water bodies, but also in temperate regions. Since this species may produce potent toxins such as cylindrospermopsin (CYN) and paralytic shellfish poisons, its massive occurrence in water bodies used as drinking water sources or for recreation is of major concern. The proliferation of C. raciborskii in German water bodies has been documented for the past decade. We investigated the occurrence of CYN in field populations and isolates of C. raciborskii from two lakes, and assessed the toxicity of culture isolates using the mouse bioassay, primary rat hepatocytes and human derived cell lines. We show for the first time the occurrence of CYN in German water bodies. None of seven isolates of C. raciborskii contained CYN, however, all isolates were toxic to primary rat hepatocytes, human hepatoblastoma (HEP-G2) and human colon adenocarcinoma (CACO-2) cells. Methanolic extracts were more toxic than aqueous extracts. Three isolates tested in the mouse bioassay were toxic at a concentration of 800 mg kg(-1) showing liver and spleen damage and inflammation of the intestine. These results give strong evidence that the German isolates of C. raciborskii contain currently not identified or unknown toxins.
Keywords: Liver; Spleen; Cells, Cultured; Tumor Cells, Cultured; Hepatocytes; Animals; Humans; Mice; Rats; Cyanobacteria; Enteritis; Uracil; Bacterial Toxins; Water Pollutants; Water Microbiology; Fresh Water; Environmental Monitoring; Water Supply; Species Specificity; Germany; Male
RMID: 0020032225
DOI: 10.1016/S0041-0101(03)00150-8
Appears in Collections:Pharmacology publications
Environment Institute publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.