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|Title:||Cytokine regulation of syndecan-1 and -2 gene expression in human periodontal fibroblasts and osteoblasts|
|Citation:||Journal of Periodontal Research, 2002; 37(4):273-278|
|Publisher:||Munksgaard Int Publ Ltd|
|Worapamorn W; Tam SP; Li H; Haase HR; Bartold PM|
|Abstract:||Cell-surface proteoglycans participate in several biological functions including interactions with a variety of growth factors and cytokines. Regulation of syndecan-1 and -2 gene expression was investigated in human periodontal ligament fibroblasts (PDLF), osteoblasts (OB) and gingival fibroblasts (GF), in response to platelet-derived growth factor (PDGF-BB), transforming growth factor (TGF-beta 1), and interleukin (IL-1 beta) by Northern blot analyses. We also compared the effect of PDGF-BB and TGF-beta 1, separately and in combination, in the prolonged presence of IL-1 beta on the expression of both syndecan genes. The results demonstrated that the three cell lines regulated the expression of syndecan-1 and -2 in response to growth factors and cytokines in different manners. These cell lines increased syndecan-1 mRNA levels in response to either PDGF-BB or TGF-beta 1 and decreased levels in response to IL-1 beta. The effect of IL-1 beta on syndecan-1 mRNA synthesis was partially reversed after adding PDGF-BB and TGF-beta 1, separately or in combination, in the presence of IL-1 beta. In contrast, syndecan-2 mRNA level was markedly upregulated in response to either TGF-beta 1 or IL-1 beta in OB when compared with the other two cell lines. However, the stimulatory effect of TGF-beta 1 on syndecan-2 mRNA production in OB was abolished in the prolonged presence of IL-1 beta. These findings lend support to the notion that syndecan-1 and syndecan-2 have distinct functions which correlate with their source and functions within the periodontium.|
|Keywords:||Alveolar Process; Fibroblasts; Osteoblasts; Gingiva; Periodontal Ligament; Humans; Proteoglycans; Platelet-Derived Growth Factor; Proto-Oncogene Proteins c-sis; Transforming Growth Factor beta; Membrane Glycoproteins; RNA, Messenger; Interleukin-1; Cytokines; Blotting, Northern; Cell Culture Techniques; Gene Expression Regulation; Up-Regulation; Transforming Growth Factor beta1; Syndecan-1; Syndecans; Syndecan-2; Statistics as Topic; Becaplermin|
|Description:||Copyright © 2002 Blackwell Munksgaard The definitive version is available at www.blackwell-synergy.com|
|Appears in Collections:||Dentistry publications|
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